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Microsystèmes pour la préparation d'échantillons sanguins

Abstract : Diagnostic tests are essentially performed on blood which is representative of complex patient pathologic states. For most analysis, the procedure to separate blood elements necessarily includes a centrifugation followed by a physical separation. So it is macroscale, manual and multi-steps, making the crucial blood fractionation step one of the last hurdles towards the total integration of blood analysis in microsystems. Thus our goal is to perform an efficient point-of-care sample preparation device to quickly, easily and reproducibly fractionate bloods elements. To do so innovating devices are firstly proposed to continuously extract plasma using only passive microfluidic effects. More precisely, these devices are based on the cell-free layer in a channel flow and its strong expansion by restriction effect and geometric singularities. For a 1:20 diluted blood injected at 100µL/min, the extraction yield is up to 17% giving a 4-fold improvement regarding a reference device. The influence of the sample dilution is analysed and extracted plasma is biologically validated. In the meantime, methods of cells sorting by affinity capture are developed, using a specific surface functionalization and antibodies arrays. A capture is set out in whole blood for each cell type, experimentally optimized and proved to be specific and proportional. Solutions have been finally proposed in view of the integration of both operations, microfluidic separation and cells sorting.
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https://tel.archives-ouvertes.fr/tel-00434954
Contributor : Elodie Sollier <>
Submitted on : Monday, November 23, 2009 - 2:41:45 PM
Last modification on : Friday, October 23, 2020 - 4:41:10 PM
Long-term archiving on: : Thursday, June 17, 2010 - 9:29:01 PM

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  • HAL Id : tel-00434954, version 1

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Elodie Sollier. Microsystèmes pour la préparation d'échantillons sanguins. Biophysique [physics.bio-ph]. Université Joseph-Fourier - Grenoble I, 2009. Français. ⟨tel-00434954⟩

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