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Theses

Cellules médullaires et biomatériaux implantables en site osseux

Abstract : Various strategies using bone marrow cells and/or biomaterials were studied for the repair of bone tissue in murine models. Among allogenic bone grafts industrially produced, some associate H2O2 and NaOH in the purification process. However, we showed that each compound induces a deterioration of matrix surface, decreasing adhesion, proliferation and activity of osteoblastic cells. NaHCO3 seemed an effective cleaning agent, without deleterious consequences on the quality of the grafts and their cytocompatibility. This process was used to produce purified bone grafts for the repair, by tissue engineering, of a calvarial bone defect of critical size in the mouse. Cells/graft hybrid construction, elaborate in vitro, induced in vivo a fast bone regeneration until the center of the defect. Induction of the osteoblast differentiation of bone marrow cells before implantation did not improve the results. Systemic transplantation of bone marrow cells was also studied. The total body irradiation of mice, a precondition to transplantation, induces a dramatic trabecular bone loss. The transplanted cells, from the bone marrow, did not allow the restoration of bone mass but reconstituted a bone microenvironnement leading to a modified microarchitecture of the recipient mice towards the donor phenotype. We have used bone marrow cells from GFP transgenic mice, allowing to trace the donor cells in wild type mice. We found that immunodepletion of CD11b expressedbone marrow cells allow to strongly enrich the cellular population in osteoprogenitors.
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https://tel.archives-ouvertes.fr/tel-00433829
Contributor : Anne-Marie Plé <>
Submitted on : Friday, November 20, 2009 - 3:01:13 PM
Last modification on : Friday, October 23, 2020 - 4:47:14 PM
Long-term archiving on: : Saturday, November 26, 2016 - 3:55:39 PM

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  • HAL Id : tel-00433829, version 1

Citation

Aline Dumas. Cellules médullaires et biomatériaux implantables en site osseux. Biologie cellulaire. Université d'Angers, 2008. Français. ⟨tel-00433829⟩

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