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Fractionnement de protéines du lait par filtration dynamique.

Abstract : This thesis focuses on milk proteins fractionation by dynamic filtration. A two-stage process has been proposed in order to separate milk proteins into three main fractions: casein micelles, α-Lactalbumin and β-Lactoglobulin. The first stage of the process consists in microfiltration of skim milk for separating casein micelles from whey proteins. The performances of two dynamic filtration modules: the Multi Shaft Disk (MSD) module and the rotating disk module have been compared. The results have shown that the MSD module presents high permeate flux, good whey proteins transmission and high casein micelles rejection. The second stage of the process is the separation of α-Lactalbumin from β-Lactoglobulin by ultrafiltration. The rotating disk module equipped with a disk with vanes and rotating at 2000 rpm, was used. The protein transmission and selectivity were constant during whey concentration. This is an advantage of dynamic filtration because in crossflow filtration protein transmission and selectivity decreased during whey concentration. The selectivities obtained with dynamic filtration, without changes in pH and ionic strength of the solution, were in the same range as those obtained with crossflow filtration after pH and ionic strength optimization.
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Contributor : Chantal Guilbert <>
Submitted on : Thursday, November 19, 2009 - 9:24:10 AM
Last modification on : Monday, September 3, 2018 - 1:33:04 PM
Long-term archiving on: : Thursday, June 17, 2010 - 8:54:57 PM


  • HAL Id : tel-00433359, version 1



V. Espina. Fractionnement de protéines du lait par filtration dynamique.. Sciences de l'ingénieur [physics]. Université de Technologie de Compiègne, 2009. Français. ⟨tel-00433359⟩



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