Abstract : The Fermi observatory was launched on June 11, 2008. It hosts the Large Area Telescope (LAT), sensitive to gamma-ray photons from 20 MeV to over 300 GeV. When the LAT began its activity, nine young and energetic pulsars were known in gamma rays. At least several tens of pulsar detections by the LAT were predicted before launch. The LAT also allowed the study of millisecond pulsars (MSPs), never firmly detected in gamma rays before Fermi. This thesis first presents the pulsar timing campaign for the LAT, in collaboration with large radiotelescopes and X-ray telescopes, allowing for high sensitivity pulsed searches. Furthermore, it lead to quasi-homogeneous coverage of the galactic MSPs, so that the search for pulsations in LAT data for this population of stars was not affected by an a priori bias. We present a search for pulsations from these objects in LAT data. For the first time, eight galactic MSPs have been detected as sources of pulsed gamma-ray emission over 100 MeV. In addition, a couple of good candidates for future detection are seen. A similar search for globular cluster MSPs has not succeeded so far. Comparison of the phase-aligned gamma-ray and radio light curves, as well as the spectral shapes, leads to the conclusion that their gamma-ray emission is similar to that of normal pulsars, and is probably produced in the outer-magnetosphere. This discovery suggests that many unresolved gamma-ray sources are unknown MSPs.