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Etudes structurales et fonctionnelles de lectines et adhésines chez Pseudomonas aeruginosa

Abstract : The opportunistic pathogen Pseudomonas aeruginosa is involved in nosocomial diseases and frequent infections in immunocompromised or cystic fibrosis patients. This bacterium is difficult to eradicate because of antibiotic resistance observed in many strains. It is able to produce a biofilm that increase this resistance. During infectious process, bacterial lectins are used to recognize and bind specifically oligosaccharides present on hosts tissues. Among them, the soluble PA-IL lectin (galactose specific) and a new identified fimbrial lectin, CupB6 are involved in adhesion and biofilm composition. The aim of this work is to characterize them biochemically and structurally. PA-IL binding site was well characterized by microcalorimtry, surface plasmon resonance and X ray crystallography, and a large number of galactose-derivative inhibitors were tested. For CupB6 we focused on the saccharide identification and demonstrated that it could be the Lewis b tetrasaccharide. Structural studies are being performed. This work takes part in a project on development of suitable glycomimetics that can be an antibiotic alternative solution.
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https://tel.archives-ouvertes.fr/tel-00430632
Contributor : Bertrand Blanchard <>
Submitted on : Monday, November 9, 2009 - 12:34:19 PM
Last modification on : Friday, November 6, 2020 - 3:54:33 AM
Long-term archiving on: : Thursday, June 17, 2010 - 5:55:53 PM

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  • HAL Id : tel-00430632, version 1

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Bertrand Blanchard. Etudes structurales et fonctionnelles de lectines et adhésines chez Pseudomonas aeruginosa. Biochimie [q-bio.BM]. Université Joseph-Fourier - Grenoble I, 2009. Français. ⟨tel-00430632⟩

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