Approches virtuelles dédiées à la technologie des puces à tissus "Tissue MicroArrays " TMA : Application à l'étude de la transformation tumorale du tissu colorectal

Redha Heus 1
1 RFMQ
TIMC-IMAG - Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525
Abstract : The recent technique of Tissue Micro Arrays "TMA" seams an essential way to investigate about the precise knowledge of tumour markers expression and their relation with the tissue architecture dynamic in the tumour development process. Briefly, this technology allows gathering, in a single block of paraffin, several hundreds of small cylindrical-shaped tissue-samples from different blocks of conventional tumour biopsies. This thesis focuses on different aspects of image processing and quality control related to TMA technology. The work is realized according to two main sections in agreement with chronological sequence of operations: design of TMA and automated analysis of TMA sections. As a first step, the concept of virtual TMA is introduced to simulate the protocols of core sampling to evaluate the TMA technology. In the second step, an original colour model associated with the chromaticity diagram xy is proposed for automatic detection of tumour markers in numerical TMA slides. The concept of sociology cell, modelled by Voronoï diagram, is finally adopted to illustrate the dynamics of the spatial distribution of cancer cells in the process of tumour development. The different methods developed in this work are applied to study the tumour markers of colorectal cancer.
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Contributor : Redha Heus <>
Submitted on : Friday, October 30, 2009 - 4:34:12 PM
Last modification on : Thursday, March 21, 2019 - 2:56:10 PM
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Redha Heus. Approches virtuelles dédiées à la technologie des puces à tissus "Tissue MicroArrays " TMA : Application à l'étude de la transformation tumorale du tissu colorectal. Sciences du Vivant [q-bio]. Université Joseph-Fourier - Grenoble I, 2009. Français. ⟨tel-00429056⟩

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