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La réorganisation de la chromatine au cours de la spermatogénèse

Abstract : During spermiogenesis, a dramatic chromatin remodelling occurs: most of the histones are replaced by small basic proteins, called transition proteins which in turn are replaced by protamines. The focus of my thesis is the charaterisation of the final chromatin organization in condensed spermatids and the mechanisms driving it. Our results show for the first time the presence of new DNA packaging structures in condensed spermatids, containing the testis specific histone variant TH2B and new histone variants identified in our laboratory. Furthermore, these new structures are specifically present in the region of pericentric heterochromatin in these cells. This reorganization seems to imply the NAP1L4 chaperone, which could be targeted to chromatin by interacting with H3 histone tails. Proteomic analysis of germinal cells allowed us to identify two others proteins, FYTTD1 and HMGB4, which don't seem to be implicated in chromatin remodelling during the late steps of spermiogenesis. Nevertheless, FYTTD1 seems to be involved in RNA processing. Since RNA could be another support for epigenetic information, the characterization of the exact role of this protein will be an exciting challenge for the future.
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Contributor : Emmanuelle Escoffier <>
Submitted on : Monday, November 9, 2009 - 9:51:19 AM
Last modification on : Friday, November 6, 2020 - 4:03:21 AM
Long-term archiving on: : Wednesday, September 22, 2010 - 1:45:08 PM


  • HAL Id : tel-00426877, version 2



Emmanuelle Escoffier. La réorganisation de la chromatine au cours de la spermatogénèse. Biologie cellulaire. Université Joseph-Fourier - Grenoble I, 2009. Français. ⟨tel-00426877v2⟩



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