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L. Montre-que, 125I]cotinine marque spécifiquement certaines structures cérébrales, comme les commissures antérieures, le corps calleux, Ces structures et d'autres (Tableau 20) ne sont pas marquées par la [3H]nicotine et la [125I]alpha-Bgt (Figure 41

N. La and . Carboxamide-cotinine-obtenue,-inhibe-comme-la-cotinine-la-fixation-de-la, 125I]cotinine (Figure 45) Dans la colonne d'affinité que nous avons synthétisée, la fonction carboxylique est amidifiée par la fonction amine du bras espaceur du gel (MATÉRIELS ET MÉTHODES) La réaction de synthèse de la N,n-propyl carboxamide cotinine imite exactement la réaction de synthèse de la colonne d'affinité. L'inhibition de la fixation de la [125I]cotinine par la N,n-propyl carboxamide cotinine souligne la faisabilité de purification des récepteurs de la cotinine par chromatographie d'affinité (Figure 46, La fixation du radioligand dans les membranes solubilisées montre que les sites récepteurs de la cotinine ont gardé l'activité de liaison, p.47

. Le-rendement-de-la-solubilisation, 50%) des sites [125I]cotinine est satisfaisant. Ces résultats suggèrent que le site récepteur de la cotinine n'est probablement pas à l'interface lipide-protéine

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