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Approche pluridisciplinaire de l'étude de l'activité biologique de particules fines

Abstract : In the industry, silicon carbide (SiC) powders, dedicated for various applications (ceramic, abrasive, etc.), are principally synthesized with the Acheson process. During this process, powders undergo several operations and treatments during which many airborne particles can be generated in the work environment and cause a risk by inhalation. Up to now, SiC toxicity has been poorly investigated with difficulties to drawn clear conclusions. In a first part, biological activity of SiC powders synthesized by Acheson process has been investigated. Then, surface particles influence on cellular response has been studied by modifying it with oxidising thermal treatment. To finish and to complete the study, we enlarged this work to SiC nanoparticles, synthesized by laser pyrolysis and sol-gel method.

To evaluate SiC particles biological activity, In Vitro assays on macrophages (RAW 264.7) have been performed by studying different fields (inflammation state, cell damages and death, oxidative stress) in relation to particles physicochemical properties (size, specific surface area, morphology, crystallographic structure, chemistry, surface state, free radical activity).
Results, presented according to a vector model, show SiC particle influence on cellular response. Industrial SiC particles produced by Acheson process are mainly characterized by a moderated activity on inflammation, no cytotoxic effect and a strong impact on oxidative stress. Differences between coarse and fine particles have been observed with a strong capacity for SiC industrial powders to generate directly free radicals (HO•, COO•-). After surface modifications by oxidising thermal treatment, cellular response evolution is principally characterized by a strong increase of inflammation and cytotoxicity levels. Moreover, a strong impact on oxidative stress is observed with SiC nanoparticles, much more important than results obtained for industrial SiC particles.
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https://tel.archives-ouvertes.fr/tel-00419902
Contributor : Andrée-Aimée Toucas <>
Submitted on : Friday, September 25, 2009 - 3:13:57 PM
Last modification on : Wednesday, June 24, 2020 - 4:18:29 PM
Long-term archiving on: : Tuesday, October 16, 2012 - 11:25:11 AM

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  • HAL Id : tel-00419902, version 1

Citation

Najih Boumahdi. Approche pluridisciplinaire de l'étude de l'activité biologique de particules fines. Génie des procédés. Ecole Nationale Supérieure des Mines de Saint-Etienne, 2009. Français. ⟨tel-00419902⟩

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