Skip to Main content Skip to Navigation
Theses

Validations biologiques et physico-chimiques d'un revêtement cellulosique de boîtes pour cultures cellulaires bioactives.

Abstract : Surface properties of biomaterials may influence protein adsorption and the composition of the protein layer may affect the morphology and the functional orientations of adherent cells. In this work, both biological and physico-chemical approaches were combined to characterize an original cellulosic coating (CEL) for cell culture and to better understand the interactions involved between a surface, proteins and finally cells. The aim of this multi-disciplinary project is to correlate surface properties (at the micrometric and at the nanometric scale) with biological activations. Three adherent murine cell lines were chosen (fibroblasts Swiss 3T3, pre-osteoblasts MC-3T3 and melanoma cells B16F10). Liquid-liquid contact angle measurements and AFM enabled to characterize the physico-chemical properties of the cellulosic substratum before and after fibronectin adsorption.

The principal results obtained with the cellulosic substratum are summerized below:
o Cell aggregation,
o A cellular proliferation inhibition with a blocking in G1-phase,
o An induction of apoptosis,
o CEL is hydrophilic and a little amount of fibronectin is adsorbed on the substratum in a conformation which is not appropriate for cell adhesion (bad accessibility to RGD site),
o Instantaneous affinity negligible of fibronectin for the cellulosic material.

This study evidences that CEL is an anti-adhesive biomaterial which gives reproducible and demonstrative results. Moreover, this work underlines the necessity to combine several approaches (ELISA assays, liquid-liquid contact angle measurements, force spectroscopy) to characterize the interaction between a protein and a biomaterial surface under physiological conditions.
Document type :
Theses
Complete list of metadatas

https://tel.archives-ouvertes.fr/tel-00410260
Contributor : Chantal Guilbert <>
Submitted on : Wednesday, August 19, 2009 - 10:16:08 AM
Last modification on : Friday, October 23, 2020 - 4:38:41 PM
Long-term archiving on: : Monday, October 15, 2012 - 4:15:29 PM

Identifiers

  • HAL Id : tel-00410260, version 1

Collections

Citation

E. Velzenberger. Validations biologiques et physico-chimiques d'un revêtement cellulosique de boîtes pour cultures cellulaires bioactives.. Sciences de l'ingénieur [physics]. Université de Technologie de Compiègne, 2008. Français. ⟨tel-00410260⟩

Share

Metrics

Record views

220

Files downloads

1058