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Développement d'algorithmes de reconstruction tomographique pour l'analyse PIXE d'échantillons biologiques

Abstract : The development of 3D microscopy techniques offering a spatial resolution of 1 μm or less has opened a large field of investigation in Cell Biology. Amongst them, an interesting advantage of ion beam micro-tomography is its ability to give quantitative results in terms of local concentrations in a direct way, using Particle Induced X-ray Emission (PIXET) combined to Scanning Transmission Ion Microscopy (STIMT) Tomography. After a brief introduction of existing reconstruction techniques, we present the principle of the DISRA code, the most complete written so far, which is the basis of the present work. We have modified and extended the DISRA algorithm by considering the specific aspects of biologic specimens. Moreover, correction procedures were added in the code to reduce noise in the tomograms. For portability purpose, a Windows graphic interface was designed to easily enter and modify experimental parameters used in the reconstruction, and control the several steps of data reduction. Results of STIMT and PIXET experiments on reference specimens and on human cancer cells will be also presented.
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Submitted on : Thursday, July 16, 2009 - 3:54:02 PM
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Duy Thuy Nguyen. Développement d'algorithmes de reconstruction tomographique pour l'analyse PIXE d'échantillons biologiques. Physique Nucléaire Théorique [nucl-th]. Université Sciences et Technologies - Bordeaux I; Université des Sciences Naturelles - Hanoi, 2008. Français. ⟨tel-00404564⟩

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