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Imagerie 3D résolue en temps pour l'aide au diagnostic médical : développement d'un système de microscopie de fluorescence multipoints sous excitation à deux photons.

Abstract : Confocal and two-photon microscopies are key methods for biomedical research and cells or tissue imaging. However, one of the main drawbacks of the conventional two-photon microscope is the imaging speed. Consequently, the first aim of this work was to speed up acquisition in order to preserve samples from too long experiments. In this context, we have developed an original set-up called multifocal multiphoton microscope composed of an optical system creating an 8×8 beam array. The second aspect of this work was to develop new methods of medical diagnosis. An early diagnosis of malignant tumors is essential to increase the therapy success and the patient's survival. But, the current standard to diagnose the presence of tumoral cells is of limited value because of its low sensitivity especially to atypical cells. Thus, the enhancement of early cancer diagnosis and treatment has involved the development of new methods to identify cancer signatures. For that purpose, we have an interest in fluorescence imaging methods for ex vivo diagnosis of cervix cancers. We then applied the same approach to detect possible resistance to drugs currently used in bladder chemotherapy. At the present time, patients attacked by a bladder cancer can be efficiently treated with combined chemotherapy such as M-VAC, but drug resistance may appear. In order to detect possible resistance to M-VAC, we have developed an ex vivo method from urinary samples, based upon the visible excitation of the drug fluorescence. Such pre-treatment step which does not exist at the present time will constitute a reliable, non constraining and inexpensive test enabling us to assess chemotherapy effectiveness.
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https://tel.archives-ouvertes.fr/tel-00391729
Contributor : Ariane Deniset-Besseau <>
Submitted on : Thursday, June 4, 2009 - 3:35:34 PM
Last modification on : Friday, October 23, 2020 - 4:58:13 PM
Long-term archiving on: : Thursday, June 10, 2010 - 8:07:35 PM

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Ariane Deniset-Besseau. Imagerie 3D résolue en temps pour l'aide au diagnostic médical : développement d'un système de microscopie de fluorescence multipoints sous excitation à deux photons.. Biophysique [physics.bio-ph]. Université Paris Sud - Paris XI, 2008. Français. ⟨tel-00391729⟩

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