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Theses

Modification des voies de repliement d'une petite protéine riche en ponts disulfure : la toxine alpha de Naja nigricollis

Abstract : Protein folding is the last stage of genetic expression. The mechanism by which proteins acquire their 3D structure remains poorly understood. During my project, I showed that the addition of one residue in one loop of the structure of toxin a from Naja nigricollis slows its oxidative folding process down in vitro. This decrease in the folding rate is due to a switch of productive pathway. NMR analysis of folding intermediates enabled me to hypothesize a structural explantation for this kinetic modification. Additionally, in order to test the influence of vectorial folding on the folding of a small disulfide-rich protein in vitro, I developed a method using antibodies raised against various parts of the reduced protein. My results showed that one of these antibodies is able to inhibit the oxidative folding of toxin a. This inhibition is reversible. Most interestingly, the use of this antibody modifies the folding pathway.
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https://tel.archives-ouvertes.fr/tel-00364212
Contributor : Celine Lentz <>
Submitted on : Wednesday, February 25, 2009 - 2:17:34 PM
Last modification on : Monday, February 10, 2020 - 6:12:52 PM
Long-term archiving on: : Tuesday, June 8, 2010 - 7:18:28 PM

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  • HAL Id : tel-00364212, version 1

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Grégori Gross. Modification des voies de repliement d'une petite protéine riche en ponts disulfure : la toxine alpha de Naja nigricollis. Sciences du Vivant [q-bio]. Museum national d'histoire naturelle - MNHN PARIS, 2008. Français. ⟨tel-00364212⟩

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