Skip to Main content Skip to Navigation
Theses

Etude du rôle de la protéine phosphatase de type 1 Glc7 dans l'inactivation des mécanismes de surveillance de l'ADN et analyse des interrégulations entre le mécanisme de surveillance de l'ADN et celui du fuseau mitotique chez la levure Saccharomyces cerevisiae.

Abstract : In eukaryotes, the proper transmission of the genetic material during cell division is partly realized through surveillance pathways (or checkpoints) which control DNA integrity and the equal repartition of the sister chromatids in the daughter cells. The DNA checkpoints are activated in cases of DNA damage or replication defects whereas the spindle checkpoint is triggered by defects altering all aspects of the spindle function. These surveillance pathways coordinate multiple responses including cell cycle arrests. They are widely conserved in eucaryotes and mutations affecting their components are frequently observed in human cancer cells.
DNA checkpoint activation has been thoroughly analyzed and involves many phosphorylation events. Cell cycle resumption following checkpoint inactivation is less understood but constitutes an essential step for cell survival. We have shown that the overexpression of the PP1 phosphatase Glc7 facilitates DNA checkpoint inactivation after DNA double-strand break in the yeast Saccharomyces cerevisiae.
The DNA and the spindle checkpoints used to be considered as independent pathways but we have shown that they are in fact interconnected. We have observed (i) that the activity of the spindle checkpoint alters the response to genotoxic stresses and (ii) that the phosphorylation state of two central components of the DNA checkpoint, Rad53 and Rad9, is modified when the spindle checkpoint is activated. We present here the characterization of these post-translational modifications along with their potential physiological significance.
Document type :
Theses
Complete list of metadatas

https://tel.archives-ouvertes.fr/tel-00361210
Contributor : Celine Lentz <>
Submitted on : Friday, February 13, 2009 - 1:16:12 PM
Last modification on : Monday, February 10, 2020 - 6:12:52 PM
Long-term archiving on: : Tuesday, June 8, 2010 - 8:46:41 PM

Identifiers

  • HAL Id : tel-00361210, version 1

Collections

Citation

Céline Clemenson. Etude du rôle de la protéine phosphatase de type 1 Glc7 dans l'inactivation des mécanismes de surveillance de l'ADN et analyse des interrégulations entre le mécanisme de surveillance de l'ADN et celui du fuseau mitotique chez la levure Saccharomyces cerevisiae.. Sciences du Vivant [q-bio]. Ecole Centrale Paris, 2007. Français. ⟨tel-00361210⟩

Share

Metrics

Record views

328

Files downloads

937