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Etude de l'ATPase cuivre eucaryote Ccc2 de Saccharomyces cerevisiae
De la localisation à la fonction

Abstract : This thesis investigated two aspects of Ccc2, S. Cerevisiae copper ATPase. One is cellular and concerns the localization and the cellular function of Ccc2; the second one is biochemical and deals with the mechanism of copper transport by Ccc2. We found out that Ccc2 localizes at all the secretory pathway compartments and at the membrane of the vacuole. The latter result is the first evidence for a new function of Ccc2, that is to store copper into the vacuole lumen. Ccc2 is the first copper active transporter found in a vacuole. Our biochemical study shows that 2 coppers atoms bind to the isolated nucleotide domain of Ccc2. Two cysteines, C708 and C718, were identify as being part of the copper binding sites. Further studies will determine the function of these copper binding sites in the mechanism of copper transport by Ccc2.
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https://tel.archives-ouvertes.fr/tel-00358960
Contributor : Simon Gudin <>
Submitted on : Thursday, February 5, 2009 - 9:28:29 AM
Last modification on : Wednesday, November 4, 2020 - 2:08:56 PM
Long-term archiving on: : Tuesday, June 8, 2010 - 9:55:22 PM

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  • HAL Id : tel-00358960, version 1

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Simon Gudin. Etude de l'ATPase cuivre eucaryote Ccc2 de Saccharomyces cerevisiae
De la localisation à la fonction. Biochimie [q-bio.BM]. Université Joseph-Fourier - Grenoble I, 2008. Français. ⟨tel-00358960⟩

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