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Analyse fonctionelle de la nouvelle famille de facteur de transcription GeBP/GPL dans le contrôle du développement d'Arabidopsis thaliana

Abstract : Our group is interested in understanding the role of plant hormones in the development process of the plant model Arabidopsis thaliana. In a search for regulators of the expression of a gene involved in the differentiation of epidermal cells whose expression is controlled by Gibberellins (GA) and Cytokinins (CK) hormones, a new family of proteins named GeBP (GL1 enhancer Binding Protein), GPL1 (GeBP Protein Like 1), GPL2 and GPL3 was identified by our team. The aim of this PhD work was to make a functional and molecular analysis of the 4 members of this new family.
First, we showed that the four GeBP/GPL proteins are nuclear localized and we demonstrated the ability of these proteins to form dimers through a new non-canonical Leucine-Zipper motif. The role of the GeBP/GPL family in plant development was assessed by studying simple mutant as well as combination of multiple mutant lines. We obtained several convergent physiological and molecular data that demonstrate a redondant role of the GeBP/GPL genes in the CK responses. This functional analysis has been completed by the transcriptomic analysis of the quadruple mutant gebp/gpl and of a surexpressor line of GPL2. This study allowed us to etablish a link between the GeBP/GPL family and CPR5, a gene involved in several processes such as pathogen responses and cell cycle. This link has been reinforced by ploïdy measurements which showed a decrease in DNA level of leaf cells of the quadruple mutant and the surexpressor of GPL2 lines as already mentioned for CPR5 mutants.
Futur works will aim at confirming the role of the GeBP/GPL in the CPR5 pathway.
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https://tel.archives-ouvertes.fr/tel-00352018
Contributor : Florian Chevalier <>
Submitted on : Monday, January 12, 2009 - 1:50:06 PM
Last modification on : Friday, November 6, 2020 - 4:09:26 AM
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  • HAL Id : tel-00352018, version 1

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Florian Chevalier. Analyse fonctionelle de la nouvelle famille de facteur de transcription GeBP/GPL dans le contrôle du développement d'Arabidopsis thaliana. Biochimie [q-bio.BM]. Université Joseph-Fourier - Grenoble I, 2008. Français. ⟨tel-00352018⟩

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