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Conséquences de la perturbation des éléments du cytosquelette dans le déroulement de la phase M et la prolifération cellulaire.

Abstract : Eukaryotic cells reproduce following a highly regulated sequence of events called the cell cycle. The cell cycle allow an accurate duplication of the genome and its subsequent separation into two identical daughter cells. Along this process, numerous checkpoint mechanisms operate to maintain the genome's integrity. Mitosis is the phase of the cell cycle where the DNA is segregated and splitted into two daughter cells. The mitotic checkpoint , or spindle assembly checkpoint, ensures the fidelity of chromosome segregation. The mitotic checkpoint delays the metaphase/anaphase transition until all the conditions for proper chromosomes segregation are set. Despite this control mecanism, cells can missegregate their chromosome and become aneuploid. This is particulary frequent in cancer cells.
In this study, we found that normal cells that undergo abnormal mitosis with chromosome missegregation arrest in the G1 phase subsequent to the abnormal mitosis and become senescent. When primary mammalians cells were treated with low doses of microtubule poisons, the spindle what partially disrupted and the rate of chromosome missalignement increased. The cells treated with low drugs became aneuploid and arrested in G1 after the abnormal mitosis. The arrest was followed by senescence. We found that cells transformed with SV40 T antigen were unable to arrest after the abnormal mitosis.
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https://tel.archives-ouvertes.fr/tel-00351834
Contributor : Stéphanie Mirabelle <>
Submitted on : Sunday, January 11, 2009 - 11:41:15 PM
Last modification on : Wednesday, November 4, 2020 - 2:05:57 PM
Long-term archiving on: : Saturday, November 26, 2016 - 4:15:44 AM

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  • HAL Id : tel-00351834, version 1

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Stéphanie Mirabelle. Conséquences de la perturbation des éléments du cytosquelette dans le déroulement de la phase M et la prolifération cellulaire.. Biologie cellulaire. Université Joseph-Fourier - Grenoble I, 2008. Français. ⟨tel-00351834⟩

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