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Règles de cohérence pour l'annotation génomique : développement et mise en oeuvre in silico et in vivo

Emmanuelle Beyne 1, 2
2 MAGNOME - Models and Algorithms for the Genome
Inria Bordeaux - Sud-Ouest, UB - Université de Bordeaux, CNRS - Centre National de la Recherche Scientifique : UMR5800
Abstract : Genome annotation identifies all significant elements located on genomic DNA, support on which the functional program for organism is written. It predicts their biological functions and their relationships. The annotation of a completely sequenced genome is subject to several constraints : it must be done quickly and represent the organism as a coherent biological functionnal system. We propose a method to check the quality of genome annotation, based on a set of coherency rules defined according to knowledge and constraints accepted by the scientific community. These rules check, on the one hand, the completeness of the annotation (presence of vital functions), and on the other hand, the absence of errors (correctness of biological sense of predicted elements). Our method is applied in the Génolevures project, a comparative genomics project within the hemiascomycetous yeasts. We developed the annotation system that eases the manual annotation work by experts. The integration of our rules within this system garantees the good quality of the resulting annotation. We further chose to validate experimentaly these rules with studying protein-protein interactions of yeasts Saccharomyces cerevisiae and Yarrowia lipolytica, using blue native and SDS polyacrylamide gel electrophoresis (BN/SDS PAGE). The results offer both new biological knowledge for the studied yeasts and a demonstration of the universality of certain rules as well as the well-founded of the annotation strategy.
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  • HAL Id : tel-00350902, version 1

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Emmanuelle Beyne. Règles de cohérence pour l'annotation génomique : développement et mise en oeuvre in silico et in vivo. Autre [cs.OH]. Université Sciences et Technologies - Bordeaux I, 2008. Français. ⟨tel-00350902⟩

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