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Toxicité et clairance pulmonaires des nanotubes de carbone

Abstract : Constituted only by carbon atoms, carbon nanotubes (CNT) are hydrophobic and hardly detectable in biological tissues. These properties make biokinetics and toxicology studies more complex. In this work, we propose to study the effect of bovine serum albumin as a dispersing agent for CNT. The toxicological effect of a multi walled CNT (MWCNT) and a method to investigate the biopersistence of CNT in organism, based on detection of nickel, a metallic impurity present in the MWCNT we investigated were then analyses. Our results, in rats that received 100 μg of MWCNT by a unique intratracheal instillation, reveal that MWCNT do not induce histopathological lesions, modifications of physiological pulmonary parameters and inflammation. Moreover, MWCNT do not significantly cross the pulmonary barrier and can be eliminated by a slow mechanism. Using electonic microscopy and infrared spectroscopy, we showed that MWCNT may be cleaved and chemically modified in the lung. Analyses of MWCNT lung clearance mechanism lead us to propose a hypothesis based on the
phagocytosis, apoptosis of alveolar macrophages, possible degradation of MWCNT by alveolar macrophages, and elimination of apoptotic cells.
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Contributor : Dan Elgrabli Connect in order to contact the contributor
Submitted on : Tuesday, December 16, 2008 - 3:37:30 PM
Last modification on : Tuesday, December 16, 2008 - 3:46:11 PM
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  • HAL Id : tel-00347713, version 1



Dan Elgrabli. Toxicité et clairance pulmonaires des nanotubes de carbone. Sciences du Vivant [q-bio]. Université Paris-Diderot - Paris VII, 2008. Français. ⟨tel-00347713⟩



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