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Analyse structurale et fonctionnelle d'une protéine LEA (Late Embryogenesis Abundant) mitochondriale exprimée dans les graines de pois

Abstract : Although few organisms are desiccation tolerant, this remarkable property is widespread among plant seeds. Survival without water relies on an array of mechanisms, including the accumulation of stress proteins such as the late embryogenesis abundant (LEA) proteins. We show here that LEAM, a mitochondrial LEA protein expressed in seeds, is a natively unfolded protein, which reversibly folds into alpha-helices upon desiccation. LEAM interacts with membranes in the dry state, especially at the level of the phosphate group of phospholipids. These interactions, which are dependent upon lipid composition, provide a protection to liposomes, against desiccation and freezing. The overall results provide strong evidence that LEAM protects the inner mitochondrial membrane during desiccation. A reverse genetic approach was engaged to confirm this axiom and precise the physiological role of LEAM.
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https://tel.archives-ouvertes.fr/tel-00346312
Contributor : Anne-Marie Plé <>
Submitted on : Thursday, December 11, 2008 - 1:10:54 PM
Last modification on : Friday, October 23, 2020 - 4:37:40 PM
Long-term archiving on: : Tuesday, June 8, 2010 - 4:22:01 PM

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  • HAL Id : tel-00346312, version 1
  • PRODINRA : 250102

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Dimitri Tolleter. Analyse structurale et fonctionnelle d'une protéine LEA (Late Embryogenesis Abundant) mitochondriale exprimée dans les graines de pois. Biologie cellulaire. Université d'Angers, 2007. Français. ⟨tel-00346312⟩

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