Skip to Main content Skip to Navigation
Theses

Suivi de la fonctionnalité des jonctions communicantes par la technique de gap-FRAP sur des modèles in vitro (2-D, 3-D) et ex vivo : Intérêt pour le diagnostic du cancer

Abstract : Assessing gap junctions functionality using gap-FRAP technique on models in vitro (2-D, 3-D) and ex vivo : Interest for cancer diagnosis Optical methods to characterize tissues for tumor detection are an area of diagnosis research. Gap-FRAP (Fluorescence recovery after photobleaching) technique is used to estimate gap junctions functionality. The majority of malignant cells are characterized by a modification of the number and functionality of gap junctions, compared to their healthy counterparts. Gap- FRAP technique appears adapted to distinguish cancerous cells from normal cells according to their intercellular communication (GJIC). Particularly used in vitro, we optimized the use of gap-FRAP technique for a potential transfer on tissues and organs ex vivo. We resolved, using cell lines with a different connexin expression and distribution pattern, the choice of a fluorescent dye for further in vivo measurements: calcein-AM. The following stage consisted in developing a system engineering model for the comparative analysis of the fluorescence recovery data for bi-dimensional and three-dimensional (spheroid) models. The conditions previously defined have been transposed in entire organ, bladder rat. Multi-color staining was applied using a fluorescent dye to track to tumors cells in bladder ex vivo, a fluorescent marker to distinguish cellular details of the entire urothelium, and calcein-AM to assess GJIC.
Gap-FRAP was used for the first time to differentiate intercellular communication degree between healthy and tumor tissue in entire organ ex vivo opening perspectives for the diagnosis of the bladder cancer correlated to feature modifications of GJIC.
Document type :
Theses
Complete list of metadatas

https://tel.archives-ouvertes.fr/tel-00343150
Contributor : Didier Wolf <>
Submitted on : Saturday, November 29, 2008 - 6:02:18 PM
Last modification on : Friday, October 23, 2020 - 4:52:31 PM
Long-term archiving on: : Monday, June 7, 2010 - 10:09:27 PM

Identifiers

  • HAL Id : tel-00343150, version 1

Collections

Citation

Muriel Abbaci. Suivi de la fonctionnalité des jonctions communicantes par la technique de gap-FRAP sur des modèles in vitro (2-D, 3-D) et ex vivo : Intérêt pour le diagnostic du cancer. Sciences du Vivant [q-bio]. Université Henri Poincaré - Nancy I, 2008. Français. ⟨tel-00343150⟩

Share

Metrics

Record views

521

Files downloads

3562