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Theses

L'influence des circulations hydrologiques sur la structure des communautés bactériennes à l'interface sol-nappe

Abstract : Water composition, relative to biological active elements such as nitrates is controlled by microbial processes that are dependent of in situ physical and chemical conditions. Chemical conditions and bacterial community structures are intimately linked but hydrological circulations effect on microbial diversity is rather poorly constrained. This becomes important since hydrological circulation that depends on landscape settings is important in determining biochemical processes. Hydrological fluxes and functioning ecosystems relationships are here discussed in 3 parts: (i) Influence of vertical fluxes on nitrates reducing bacterial community structure (narG gene); (ii) Influence of vertical fluxes on integrative (ARNr 16S gene) and functioning (narG gene) bacterial community structure; and (iii) Influence of forcing parameters on proteins expression (Metaproteomic approach).
The first part is carried out using bioreactors simulating different redox conditions and nutriment availability. The second part consists in monitoring changes in the community structure through time (high and low water table periods) and depth. Finally the third part experimentally deals with varying forcing parameters (amount and nature of carbon, and amount of nitrogen) influence on water sampled at high depth. For each bioreactor, a peptidic fingerprint (metaproteomic analyze) is performed to simulate functioning ecosystem.
Hydrological circulation influence on bacterial community is confirmed with a direct effect on community structure and an indirect outcome via resources availability. Cells proteomes exposed to two Carbone sources appear significantly different, suggesting a system specific response to the nature of an induced pollution.
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https://tel.archives-ouvertes.fr/tel-00342702
Contributor : Isabelle Dubigeon <>
Submitted on : Friday, November 28, 2008 - 11:31:54 AM
Last modification on : Monday, April 29, 2019 - 2:30:44 PM
Long-term archiving on: : Monday, June 7, 2010 - 10:07:21 PM

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  • HAL Id : tel-00342702, version 1

Citation

Nolwenn Bougon. L'influence des circulations hydrologiques sur la structure des communautés bactériennes à l'interface sol-nappe. Hydrologie. Université Rennes 1, 2007. Français. ⟨tel-00342702⟩

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