T. Devolder, B. Garthoff, K. Muller, M. Pauwels, V. Rogiers et al., The impact of REACH, The report of CONAM / ecopa Chemical Policy Working Group, 2007.

M. Balls, A. Goldberg, J. Fentem, C. Broadhead, R. Burch et al., The three Rs: the way forward: the report and recommendations of ECVAM Workshop 11, Altern Lab Anim, vol.23, pp.838-866, 1995.

D. Smith and W. Van-de, Pharmacokinetics and metabolism in early drug discovery, Current Opinion in Chemical Biology, vol.3, issue.4, pp.373-378, 1999.
DOI : 10.1016/S1367-5931(99)80056-8

K. Pang, MODELING OF INTESTINAL DRUG ABSORPTION: ROLES OF TRANSPORTERS AND METABOLIC ENZYMES (FOR THE GILLETTE REVIEW SERIES), Drug Metabolism and Disposition, vol.31, issue.12, pp.1507-1519, 2003.
DOI : 10.1124/dmd.31.12.1507

J. Lin, M. Chiba, and T. Baillie, Is the role of the small intestine in first-pass metabolism overemphasized?, Pharmacol Rev, vol.51, pp.135-158, 1999.

J. Lin and A. Lu, Role of pharmacokinetics and metabolism in drug discovery and development, Pharmacol Rev, vol.49, pp.403-449, 1997.

H. Rang, M. Dale, J. Ritter, and R. Flower, Rang & Dale's Pharmacology, 2007.

B. Pleuvry, Factors affecting drug absorption and distribution, Anaesthesia & Intensive Care Medicine, vol.6, issue.4, pp.135-138, 2005.
DOI : 10.1383/anes.6.4.135.63632

Y. Kurosaki and T. Kimura, Regional Variation in Oral Mucosal Drug Permeability, Critical Reviews?? in Therapeutic Drug Carrier Systems, vol.17, issue.5, pp.467-508, 2000.
DOI : 10.1615/CritRevTherDrugCarrierSyst.v17.i5.20

P. Wertz and C. Squier, Cellular and molecular basis of barrier function in oral epithelium, Crit Rev Ther Drug Carrier Syst, vol.8, pp.237-269, 1991.

H. Barennes, T. Balima-koussoube, N. Nagot, J. Charpentier, and E. Pussard, Safety and efficacy of rectal compared with intramuscular quinine for the early treatment of moderately severe malaria in children: randomised clinical trial, BMJ, vol.332, issue.7549, pp.1055-1059, 2006.
DOI : 10.1136/bmj.332.7549.1055

J. Jantzen and P. Diehl, Rectal administration of drugs. Fundamentals and applications in anesthesia, Anaesthesist, vol.40, pp.251-261, 1991.

A. De-boer, D. Breimer, J. Gubbens-stibbe, and A. Bosma, First-pass elimination of salicylamide in man following oral and rectal administration, Biopharmaceutics & Drug Disposition, vol.32, issue.4, pp.321-330, 1983.
DOI : 10.1002/bdd.2510040404

A. De-boer, F. Moolenaar, L. De-leede, and D. Breimer, Rectal Drug Administration, Clinical Pharmacokinetics, vol.7, issue.4, pp.285-311, 1982.
DOI : 10.2165/00003088-198207040-00002

. Wadell-c, Nasal drug delivery : in vitro studies on factors influencing permeability and implications on absorption, Thèse, vol.55, 2002.

A. Hussain and F. Ahsan, The vagina as a route for systemic drug delivery, Journal of Controlled Release, vol.103, issue.2, pp.301-313, 2005.
DOI : 10.1016/j.jconrel.2004.11.034

N. Das and M. Bahia, Gels as vaginal drug delivery systems, Int J Pharm, vol.318, pp.1-14, 2006.

B. Van-der and A. Van-eyk, Comparative in vitro permeability of human vaginal, small intestinal and colonic mucosa, International Journal of Pharmaceutics, vol.261, issue.1-2, pp.147-152, 2003.
DOI : 10.1016/S0378-5173(03)00298-9

P. Mills and S. Cross, Transdermal drug delivery: Basic principles for the veterinarian, The Veterinary Journal, vol.172, issue.2, pp.218-233, 2006.
DOI : 10.1016/j.tvjl.2005.09.006

J. Krause, Krause's essential human histology for medical students, Livre, 2005.

S. Tsukita and M. Furuse, Claudin-based barrier in simple and stratified cellular sheets, Current Opinion in Cell Biology, vol.14, issue.5, pp.531-536, 2002.
DOI : 10.1016/S0955-0674(02)00362-9

E. Dejana, M. Corada, and M. Lampugnani, Endothelial cell-to-cell junctions, FASEB J, vol.9, pp.910-918, 1995.

S. Liebner, U. Cavallaro, and E. Dejana, The Multiple Languages of Endothelial Cell-to-Cell Communication, Arteriosclerosis, Thrombosis, and Vascular Biology, vol.26, issue.7, pp.1431-1438, 2006.
DOI : 10.1161/01.ATV.0000218510.04541.5e

J. Mullin, N. Agostino, E. Rendon-huerta, and J. Thornton, Keynote review: Epithelial and endothelial barriers in human disease???, Drug Discovery Today, vol.10, issue.6, pp.395-408, 2005.
DOI : 10.1016/S1359-6446(05)03379-9

M. Gilcrease, Integrin signaling in epithelial cells, Cancer Letters, vol.247, issue.1, pp.1-25, 2007.
DOI : 10.1016/j.canlet.2006.03.031

C. Scully, J. Bagan, M. Black, M. Carrozzo, D. Eisen et al., Epithelial biology, Oral Diseases, vol.109, issue.Suppl., pp.58-71, 2005.
DOI : 10.1111/j.1601-0825.2004.01078.x

A. Shojaei, Buccal mucosa as a route for systemic drug delivery: a review, J Pharm Pharm Sci, vol.1, pp.15-30, 1998.

T. Ann, Pulmonary drug absorption : in vitro investigations of drug absorption across the lung barrier and its relation to drug physicochemical properties, Thèse, vol.86, 2002.

C. Ehrhardt, J. Fiegel, S. Fuchs, R. Bu-dahab, U. Schaefer et al., Drug Absorption by the Respiratory Mucosa: Cell Culture Models and Particulate Drug Carriers, Journal of Aerosol Medicine, vol.15, issue.2, pp.131-139, 2002.
DOI : 10.1089/089426802320282257

A. Hussain, J. Arnold, M. Khan, and F. Ahsan, Absorption enhancers in pulmonary protein delivery, Journal of Controlled Release, vol.94, issue.1, pp.15-24, 2004.
DOI : 10.1016/j.jconrel.2003.10.001

A. Bishop, Pulmonary epithelial stem cells, Cell Proliferation, vol.44, issue.38, pp.89-96, 2004.
DOI : 10.1016/0300-483X(76)90046-9

A. Ismail and L. Hooper, Epithelial Cells and Their Neighbors. IV. Bacterial contributions to intestinal epithelial barrier integrity, AJP: Gastrointestinal and Liver Physiology, vol.289, issue.5, pp.779-784, 2005.
DOI : 10.1152/ajpgi.00203.2005

D. Powell, Barrier function of epithelia, Am J Physiol, vol.241, pp.275-288, 1981.

P. Tuma and A. Hubbard, Transcytosis: Crossing Cellular Barriers, Physiological Reviews, vol.83, issue.3, pp.871-932, 2003.
DOI : 10.1152/physrev.00001.2003

M. Morris, H. Lee, and L. Predko, Gender Differences in the Membrane Transport of Endogenous and Exogenous Compounds, Pharmacological Reviews, vol.55, issue.2, pp.229-240, 2003.
DOI : 10.1124/pr.55.2.1

N. Mizuno, T. Niwa, Y. Yotsumoto, and Y. Sugiyama, Impact of Drug Transporter Studies on Drug Discovery and Development, Pharmacological Reviews, vol.55, issue.3, pp.425-461, 2003.
DOI : 10.1124/pr.55.3.1

R. Jones, The pharmacology of cocaine smoking in humans, NIDA Res Monogr, vol.99, pp.30-41, 1990.
DOI : 10.1037/e496372006-004

T. Fournier, N. Medjoubi, and D. Porquet, Alpha-1-acid glycoprotein, Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, vol.1482, issue.1-2, pp.157-171, 2000.
DOI : 10.1016/S0167-4838(00)00153-9

O. Henry, J. Blacher, J. Verdavaine, M. Duviquet, and M. Safar, Alpha 1-acid glycoprotein is an independent predictor of in-hospital death in the elderly, Age and Ageing, vol.32, issue.1, pp.37-42, 2003.
DOI : 10.1093/ageing/32.1.37

Y. Gueguen, K. Mouzat, L. Ferrari, E. Tissandie, J. Lobaccaro et al., Cytochromes P450: xenobiotic metabolism, regulation and clinical importance, Ann Biol Clin, vol.64, pp.535-548, 2006.

E. Levesque, D. Hum, and A. Belanger, R??le des UDP-glucuronosyltransf??rases (UGT) dans le m??tabolisme des hormones st??ro??diennes., m??decine/sciences, vol.17, issue.1, pp.33-43, 2001.
DOI : 10.4267/10608/1785

F. Lakehal, D. Wendum, V. Barbu, L. Becquemont, R. Poupon et al., Phase I and Phase II drug-metabolizing enzymes are expressed and heterogeneously distributed in the biliary epithelium, Hepatology, vol.90, issue.6, pp.1498-1506, 1999.
DOI : 10.1002/hep.510300619

J. Hukkanen, Xenobiotic-metabolizing cytochrome P450 enzymes in human lung, Thèse, vol.69, 2001.

E. Lecluyse, Human hepatocyte culture systems for the in vitro evaluation of cytochrome P450 expression and regulation, European Journal of Pharmaceutical Sciences, vol.13, issue.4, pp.343-368, 2001.
DOI : 10.1016/S0928-0987(01)00135-X

V. Catania, S. Pozzi, E. Luquita, M. Ruiz, M. Villanueva et al., Co-regulation of expression of phase II metabolizing enzymes and multidrug resistance-associated protein 2, Ann Hepatol, vol.3, pp.11-17, 2004.

O. Brien, P. Chan, K. Silber, and P. , Human and animal hepatocytes in vitro with extrapolation in vivo, Chem Biol Interact, vol.150, pp.97-114, 2004.

A. Radominska-pandya, P. Czernik, J. Little, E. Battaglia, and P. Mackenzie, STRUCTURAL AND FUNCTIONAL STUDIES OF UDP-GLUCURONOSYLTRANSFERASES*, Drug Metabolism Reviews, vol.26, issue.4, pp.817-899, 1999.
DOI : 10.1006/bbrc.1997.7388

M. Shipkova and E. Wieland, Glucuronidation in therapeutic drug monitoring, Clinica Chimica Acta, vol.358, issue.1-2, pp.2-232, 2005.
DOI : 10.1016/j.cccn.2005.02.023

P. Van-bladeren, Glutathione conjugation as a bioactivation reaction, Chemico-Biological Interactions, vol.129, issue.1-2, pp.61-76, 2000.
DOI : 10.1016/S0009-2797(00)00214-3

M. Habdous, G. Siest, B. Herbert, M. Vincent-viry, and S. Visvikis, Polymorphismes des glutathio Stransférases et pathologies humaines : bilan des études épidémiologiques, Ann Biol Clin, vol.62, pp.15-24, 2004.

N. Hewitt, K. De, and E. Lecluyse, Induction of drug metabolizing enzymes: A survey of in vitro methodologies and interpretations used in the pharmaceutical industry???Do they comply with FDA recommendations?, Chemico-Biological Interactions, vol.168, issue.1, pp.51-65, 2007.
DOI : 10.1016/j.cbi.2006.12.009

M. Donato, J. Castell, and M. Gomez-lechon, Characterization of drug metabolizing activities in pig hepatocytes for use in bioartificial liver devices: comparison with other hepatic cellular models, Journal of Hepatology, vol.31, issue.3, pp.542-549, 1999.
DOI : 10.1016/S0168-8278(99)80049-X

P. Taavitsainen, Cytochrome P450 isoform-specific in vitro methods to predict drug metabolism and interactions, Thèse, vol.67, 2001.

M. Huang, R. Chang, J. Fortner, and A. Conney, Studies on the mechanism of activation of microsomal Benzo[a]pyrene hydroxylation by Flavonoids, The Journal of Biological Chemistry, vol.256, pp.6829-6836, 1981.

M. Turpeinen, Cytochrome P450 enzymes -in vitro, in vivo, and in silico studies, Thèse, vol.102, 2006.

J. Miners, R. Mckinnon, and P. Mackenzie, Genetic polymorphisms of UDPglucuronosyltransferases and their functional significance, Toxicology, pp.181-182453, 2002.

M. Ingelman-sundberg, M. Oscarson, and R. Mclellan, Polymorphic human cytochrome P450 enzymes: an opportunity for individualized drug treatment, Trends in Pharmacological Sciences, vol.20, issue.8, pp.342-349, 1999.
DOI : 10.1016/S0165-6147(99)01363-2

Y. Ando, H. Saka, G. Asai, S. Sugiura, K. Shimokata et al., UGT1A1 genotypes and glucuronidation of SN-38, the active metabolite of irinotecan, Annals of Oncology, vol.9, issue.8, pp.845-847, 1998.
DOI : 10.1023/A:1008438109725

J. Hukkanen, J. P. , I. Benowitz, and N. , Metabolism and Disposition Kinetics of Nicotine, Pharmacological Reviews, vol.57, issue.1, pp.79-115, 2005.
DOI : 10.1124/pr.57.1.3

D. Sica, Interaction of Grapefruit Juice and Calcium Channel Blockers, American Journal of Hypertension, vol.19, issue.7, pp.768-773, 2006.
DOI : 10.1016/j.amjhyper.2005.11.003

B. Rouveix, Antibiotic Safety Assessment, International Journal of Antimicrobial Agents, vol.21, issue.3, pp.215-221, 2003.
DOI : 10.1016/S0924-8579(02)00354-0

M. Tingle and N. Helsby, Can in vitro drug metabolism studies with human tissue replace in vivo animal studies?, Environmental Toxicology and Pharmacology, vol.21, issue.2, pp.184-190, 2006.
DOI : 10.1016/j.etap.2005.07.008

A. Sivaraman, A microfabricated 3D tissue engineeringLiver on a chip":High information content assays for in vitro drug metabolism studies, Thèse, 2004.

B. David, Mise en place et validation d'un modèle in vitro pour l'étude des propriétés mécaniques, diffusives et métaboliques d'un foie bioartificiel à lit fluidisé, Thèse, 2002.

A. Kierszenbaum, Histologie et biologie cellulaire Une introduction à l'anatomie pathologique, Livre, vol.638, 2006.

B. Pleuvry, Modes of drug elimination, Anaesthesia & Intensive Care Medicine, vol.6, issue.8, pp.277-279, 2005.
DOI : 10.1383/anes.2005.6.8.277

C. Brochot, Paramétrisation des modeles physiologiques toxico/parmacocinétiques, Thèse, vol.160, 2004.

R. Fearn and B. Hirst, Predicting oral drug absorption and hepatobiliary clearance: Human intestinal and hepatic in vitro cell models, Environmental Toxicology and Pharmacology, vol.21, issue.2, pp.168-178, 2006.
DOI : 10.1016/j.etap.2005.06.002

M. Adams, L. Holland, and P. Bostwick, Pharmacology for nurses: A pathophysiologic approach, 2008.

S. Ekins, J. Mestres, and B. Testa, pharmacology for drug discovery: methods for virtual ligand screening and profiling, British Journal of Pharmacology, vol.49, issue.1, pp.9-20, 2007.
DOI : 10.1038/sj.bjp.0707305

J. Macgregor, J. Collins, Y. Sugiyama, C. Tyson, J. Dean et al., In Vitro Human Tissue Models in Risk Assessment: Report of a Consensus-Building Workshop, Toxicological Sciences, vol.59, issue.1, pp.17-36, 2001.
DOI : 10.1093/toxsci/59.1.17

C. Hillier and D. Bunton, Functional human tissue assays, Drug Discovery Today, vol.12, issue.9-10, pp.382-388, 2007.
DOI : 10.1016/j.drudis.2007.03.012

G. Cezar, Can human embryonic stem cells contribute to the discovery of safer and more effective drugs?, Current Opinion in Chemical Biology, vol.11, issue.4, pp.405-409, 2007.
DOI : 10.1016/j.cbpa.2007.05.033

T. Operana, N. Nguyen, S. Chen, D. Beaton, and R. Tukey, Human CYP1A1GFP Expression in Transgenic Mice Serves as a Biomarker for Environmental Toxicant Exposure, Toxicological Sciences, vol.95, issue.1, pp.98-107, 2007.
DOI : 10.1093/toxsci/kfl144

F. Gonzalez and C. Cheung, Humanized mouse lines and their application for prediction of human drug metabolism and toxicological risk assessment, J Pharmacol Exp Ther, 2008.

W. Zhang, A. Purchio, K. Chen, J. Wu, L. Lu et al., A TRANSGENIC MOUSE MODEL WITH A LUCIFERASE REPORTER FOR STUDYING IN VIVO TRANSCRIPTIONAL REGULATION OF THE HUMAN CYP3A4 GENE, Drug Metabolism and Disposition, vol.31, issue.8, pp.1054-1064, 2003.
DOI : 10.1124/dmd.31.8.1054

E. Brandon, C. Raap, I. Meijerman, J. Beijnen, and J. Schellens, An update on in vitro test methods in human hepatic drug biotransformation research: pros and cons, Toxicology and Applied Pharmacology, vol.189, issue.3, pp.233-246, 2003.
DOI : 10.1016/S0041-008X(03)00128-5

H. Lennernas, Animal data: The contributions of the Ussing Chamber and perfusion systems to predicting human oral drug delivery in vivo???, Advanced Drug Delivery Reviews, vol.59, issue.11, pp.1103-1120, 2007.
DOI : 10.1016/j.addr.2007.06.016

N. Katsura, I. Ikai, T. Mitaka, T. Shiotani, S. Yamanokuchi et al., Long-Term Culture of Primary Human Hepatocytes with Preservation of Proliferative Capacity and Differentiated Functions, Journal of Surgical Research, vol.106, issue.1, pp.115-23115, 2002.
DOI : 10.1006/jsre.2002.6446

A. Li, Human hepatocytes: Isolation, cryopreservation and applications in drug development, Chemico-Biological Interactions, vol.168, issue.1, pp.16-29, 2007.
DOI : 10.1016/j.cbi.2007.01.001

L. Pichard, E. Raulet, G. Fabre, J. Ferrini, J. Ourlin et al., Human Hepatocyte Culture, Methods Mol Biol, vol.320, pp.283-293, 2006.
DOI : 10.1385/1-59259-998-2:283

A. Li and M. Jurima-romet, Applications of primary human hepatocytes in the evaluation of pharmacokinetic drug-drug interactions: evaluation of model drugs terfenadine and rifampin, Cell Biology and Toxicology, vol.13, issue.4/5, pp.365-374, 1997.
DOI : 10.1023/A:1007451911843

H. Chen, H. Wu, C. Fon, P. Chen, M. Lai et al., Long-term culture of hepatocytes from human adults, Journal of Biomedical Science, vol.81, issue.6, pp.435-440, 1998.
DOI : 10.1007/BF02255932

S. Ekins, B. Ring, J. Grace, D. Robie-belle, and S. Wrighton, Present and future in vitro approaches for drug metabolism, Journal of Pharmacological and Toxicological Methods, vol.44, issue.1, pp.313-324, 2000.
DOI : 10.1016/S1056-8719(00)00110-6

J. Lam and L. Benet, Hepatic microsome studies are insufficient to characterize in vivo hepatic metabolic clearance and metabolic drug-drug interactions: studies of digoxin metabolism in primary rat hepatocytes versus microsomes, Drug Metab Dispos, vol.32, pp.1311-1316, 2004.

R. De-kanter, M. Monshouwer, D. Meijer, and G. Groothuis, Precision-Cut Organ Slices as a Tool to Study Toxicity and Metabolism of Xenobiotics with Special Reference to Non-Hepatic Tissues, Current Drug Metabolism, vol.3, issue.1, pp.39-59, 2002.
DOI : 10.2174/1389200023338071

D. Farkas, Development of a collagen gel sandwich hepatocyte bioreactor for detecting hepatotoxicity of drugs and chemicals, Thèse, vol.174, 2004.

H. Martin, B. Bournique, J. Sarsat, V. Albaladejo, and C. Lerche-langrand, Cryopreserved Rat Liver Slices: A Critical Evaluation of Cell Viability, Histological Integrity, and Drug-Metabolizing Enzymes, Cryobiology, vol.41, issue.2, pp.135-144, 2000.
DOI : 10.1006/cryo.2000.2275

G. De, I. Draaisma, A. Schoeman, O. Fahy, G. Groothuis et al., Cryopreservation of rat precision-cut liver and kidney slices by rapid freezing and vitrification, Cryobiology Feb, vol.5454, issue.1, pp.1-121, 2006.

G. De, I. Draaisma, A. Schoeman, O. Fahy, G. Groothuis et al., Cryopreservation of rat precision-cut liver and kidney slices by rapid freezing and vitrification, Cryobiology Feb, vol.5454, issue.1, pp.1-121, 2006.

A. Guillouzo, Liver cell models in in vitro toxicology, Environmental Health Perspectives, vol.106, issue.Suppl 2, pp.511-532, 1999.
DOI : 10.1289/ehp.98106511

D. Roymans, P. Annaert, H. Van, A. Weygers, J. Noukens et al., EXPRESSION AND INDUCTION POTENTIAL OF CYTOCHROMES P450 IN HUMAN CRYOPRESERVED HEPATOCYTES, Drug Metabolism and Disposition, vol.33, issue.7, pp.1004-1016, 2005.
DOI : 10.1124/dmd.104.003046

F. Zucco, A. De, I. Testai, E. Stammati, and A. , Toxicology investigations with cell culture systems: 20 years after, Toxicology in Vitro, vol.18, issue.2, pp.153-163, 2004.
DOI : 10.1016/S0887-2333(03)00147-4

M. Sawada and T. Kamataki, Genetically engineered cells stably expressing cytochrome P450 and their application to mutagen assays, Mutation Research/Reviews in Mutation Research, vol.411, issue.1, pp.19-43, 1998.
DOI : 10.1016/S1383-5742(98)00005-2

S. Coecke, A. Bogni, I. Langezaal, A. Worth, T. Hartung et al., The use of genetically engineered cells for assessing CYP2D6-related polymorphic effects, Toxicology in Vitro, vol.15, issue.4-5, pp.553-556, 2001.
DOI : 10.1016/S0887-2333(01)00061-3

P. Gripon, S. Rumin, S. Urban, S. Le, D. Glaise et al., Nonlinear partial differential equations and applications: Infection of a human hepatoma cell line by hepatitis B virus, Proceedings of the National Academy of Sciences, vol.99, issue.24, pp.15655-15660, 2002.
DOI : 10.1073/pnas.232137699

A. Guillouzo, A. Corlu, C. Aninat, D. Glaise, F. Morel et al., The human hepatoma HepaRG cells: A highly differentiated model for studies of liver metabolism and toxicity of xenobiotics, Chemico-Biological Interactions, vol.168, issue.1, pp.66-73, 2007.
DOI : 10.1016/j.cbi.2006.12.003

J. Davila, G. Cezar, M. Thiede, S. Strom, T. Miki et al., Use and Application of Stem Cells in Toxicology, Toxicological Sciences, vol.79, issue.2, pp.214-223, 2004.
DOI : 10.1093/toxsci/kfh100

L. Casteilla and C. Dani, Adipose tissue-derived cells: from physiology to regenerative medicine, Diabetes & Metabolism, vol.32, issue.5, pp.393-401, 2006.
DOI : 10.1016/S1262-3636(07)70297-5

URL : https://hal.archives-ouvertes.fr/hal-00304330

S. Snykers, T. Vanhaecke, P. Papeleu, A. Luttun, Y. Jiang et al., Sequential Exposure to Cytokines Reflecting Embryogenesis: The Key for in vitro Differentiation of Adult Bone Marrow Stem Cells into Functional Hepatocyte-like Cells, Toxicological Sciences, vol.94, issue.2, pp.330-341, 2006.
DOI : 10.1093/toxsci/kfl058

J. Mcneish, Stem cells as screening tools in drug discovery, Current Opinion in Pharmacology, vol.7, issue.5, pp.515-520, 2007.
DOI : 10.1016/j.coph.2007.06.005

K. Rodolfa, D. Giorgio, F. Sullivan, and S. , Defined reprogramming: a vehicle for changing the differentiated state, Differentiation, vol.75, issue.7, pp.577-579, 2007.
DOI : 10.1111/j.1432-0436.2007.00213.x

P. Pascal, F. Bensiam, C. Auxenfans, M. Callu, O. Robert et al., Droit concernant les tissus humains ?? finalit?? de recherche, Pathologie Biologie, vol.53, issue.10, pp.571-575, 2005.
DOI : 10.1016/j.patbio.2004.12.017

R. Le, M. De, J. Di-donato, L. Boccon-gibod, P. Teillac et al., Collections of human biological resources for research purposes: from regulations to the need of a guide of good collection practices, Rev Med Interne, vol.24, pp.803-814, 2003.

K. Bhadriraju and C. Chen, Engineering cellular microenvironments to improve cell-based drug testing, Drug Discovery Today, vol.7, issue.11, pp.612-620, 2002.
DOI : 10.1016/S1359-6446(02)02273-0

H. Takashi, M. Katsumi, and A. Toshihiro, Hepatocytes maintain their function on basement membrane formed by epithelial cells, Biochemical and Biophysical Research Communications, vol.359, issue.1, 2007.
DOI : 10.1016/j.bbrc.2007.05.079

M. Griffiths, S. Keir, and A. Burt, Basement membrane proteins in the space of Disse: a reappraisal., Journal of Clinical Pathology, vol.44, issue.8, pp.646-648, 1991.
DOI : 10.1136/jcp.44.8.646

L. Hansen, D. Mooney, J. Vacanti, and D. Ingber, Integrin binding and cell spreading on extracellular matrix act at different points in the cell cycle to promote hepatocyte growth., Molecular Biology of the Cell, vol.5, issue.9, pp.967-975, 1994.
DOI : 10.1091/mbc.5.9.967

N. Hewitt, K. Buhring, J. Dasenbrock, J. Haunschild, B. Ladstetter et al., Studies comparing in vivo:in vitro metabolism of three pharmaceutical compounds in rat, dog, monkey, and human using cryopreserved hepatocytes, microsomes, and collagen gel immobilized hepatocyte cultures, Drug Metab Dispos, vol.29, pp.1042-1050, 2001.

C. Shen, G. Zhang, H. Qiu, and Q. Meng, Acetaminophen-induced hepatotoxicity of gel entrapped rat hepatocytes in hollow fibers, Chemico-Biological Interactions, vol.162, issue.1, pp.53-61, 2006.
DOI : 10.1016/j.cbi.2006.05.005

S. Ng, R. Han, S. Chang, J. Ni, W. Hunziker et al., Improved Hepatocyte Excretory Function by Immediate Presentation of Polarity Cues, Tissue Engineering, vol.12, issue.8, pp.2181-2191, 2006.
DOI : 10.1089/ten.2006.12.2181

T. Takezawa, A strategy for the development of tissue engineering scaffolds that regulate cell behavior, Biomaterials, vol.24, issue.13, pp.2267-2275, 2003.
DOI : 10.1016/S0142-9612(03)00038-3

_. Références, T. Takezawa, T. Takenouchi, K. Imai, T. Takahashi et al., Cell culture on thin tissue sections commonly prepared for histopathology, FASEB J, vol.16, pp.1847-1849, 2002.

K. Hoebe, R. Witkamp, J. Fink-gremmels, V. Miert, A. Monshouwer et al., Direct cell-tocell contact between Kupffer cells and hepatocytes augments endotoxin-induced hepatic injury, Am J Physiol Gastrointest Liver Physiol, vol.280, pp.720-728, 2001.

S. Bhatia, U. Balis, M. Yarmush, and M. Toner, Effect of cell-cell interactions in preservation of cellular phenotype: cocultivation of hepatocytes and nonparenchymal cells, FASEB J, vol.13, pp.1883-1900, 1999.

C. Guguen-guillouzo and A. Guillouzo, Modulation of functional activities in cultured rat hepatocytes, Mol Cell Biochem, pp.53-5435, 1983.

C. Guguen-guillouzo, B. Clement, G. Baffet, C. Beaumont, E. Morel-chany et al., Maintenance and reversibility of active albumin secretion by adult rat hepatocytes co-cultured with another liver epithelial cell type, Experimental Cell Research, vol.143, issue.1, pp.47-54, 1983.
DOI : 10.1016/0014-4827(83)90107-6

M. Hirose, M. Yamato, O. Kwon, M. Harimoto, A. Kushida et al., Temperature-Responsive surface for novel co-culture systems of hepatocytes with endothelial cells: 2-D patterned and double layered co-cultures, Yonsei Medical Journal, vol.41, issue.6, pp.803-813, 2000.
DOI : 10.3349/ymj.2000.41.6.803

Y. Tsuda, A. Kikuchi, M. Yamato, G. Chen, and T. Okano, Heterotypic cell interactions on a dually patterned surface, Biochemical and Biophysical Research Communications, vol.348, issue.3, pp.937-944, 2006.
DOI : 10.1016/j.bbrc.2006.07.138

Y. Tsuda, A. Kikuchi, M. Yamato, A. Nakao, Y. Sakurai et al., The use of patterned dual thermoresponsive surfaces for the collective recovery as co-cultured cell sheets, Biomaterials, vol.26, issue.14, pp.1885-1893, 2005.
DOI : 10.1016/j.biomaterials.2004.06.005

Y. Tsuda, T. Shimizu, M. Yamato, A. Kikuchi, T. Sasagawa et al., Cellular control of tissue architectures using a three-dimensional tissue fabrication technique, Biomaterials, vol.28, issue.33, pp.4939-4946, 2007.
DOI : 10.1016/j.biomaterials.2007.08.002

H. Lu, K. Chua, P. Zhang, W. Lim, S. Ramakrishna et al., Three-dimensional co-culture of rat hepatocyte spheroids and NIH/3T3 fibroblasts enhances hepatocyte functional maintenance, Acta Biomaterialia, vol.1, issue.4, pp.399-410, 2005.
DOI : 10.1016/j.actbio.2005.04.003

S. Choi, M. Nishikawa, A. Sakoda, and Y. Sakai, Feasibility of a simple double-layered coculture system incorporating metabolic processes of the intestine and liver tissue: application to the analysis of benzo[a]pyrene toxicity, Toxicology in Vitro, vol.18, issue.3, pp.393-402, 2004.
DOI : 10.1016/j.tiv.2003.09.010

T. Morken, U. Sonnewald, M. Aschner, and T. Syversen, Effects of Methylmercury on Primary Brain Cells in Mono- and Co-culture, Toxicological Sciences, vol.87, issue.1, pp.169-175, 2005.
DOI : 10.1093/toxsci/kfi227

S. Duport, F. Robert, D. Muller, G. Grau, L. Parisi et al., An in vitro blood-brain barrier model: Cocultures between endothelial cells and organotypic brain slice cultures, Proceedings of the National Academy of Sciences, vol.95, issue.4, pp.1840-1845, 1998.
DOI : 10.1073/pnas.95.4.1840

L. Laitinen, Caco-2 cell cultures in the assessment of intestinal absorption: Effects of some coadministered drugs and natural compounds in biological matrices, Thèse, vol.73, 2006.

M. Blauer, P. Heinonen, P. Martikainen, E. Tomas, and T. Ylikomi, A novel organotypic culture model for normal human endometrium: regulation of epithelial cell proliferation by estradiol and medroxyprogesterone acetate, Human Reproduction, vol.20, issue.4, pp.864-871, 2005.
DOI : 10.1093/humrep/deh722

B. Lombardi, P. Schlupp, W. Mehnert, and M. Schafer-korting, In vitro skin absorption and drug release ??? A comparison of six commercial prednicarbate preparations for topical use, European Journal of Pharmaceutics and Biopharmaceutics, vol.68, issue.2, 2007.
DOI : 10.1016/j.ejpb.2007.05.003

S. Choi, M. Nishikawa, A. Sakoda, and Y. Sakai, Feasibility of a simple double-layered coculture system incorporating metabolic processes of the intestine and liver tissue: application to the analysis of benzo[a]pyrene toxicity, Toxicology in Vitro, vol.18, issue.3, pp.393-402, 2004.
DOI : 10.1016/j.tiv.2003.09.010

S. Schmitmeier, A. Langsch, I. Jasmund, and A. Bader, Development and characterization of a small-scale bioreactor based on a bioartificial hepatic culture model for predictive pharmacological in vitro screenings, Biotechnology and Bioengineering, vol.14, issue.6, pp.1198-1206, 2006.
DOI : 10.1002/bit.21089

C. Shen, G. Zhang, and Q. Meng, An in vitro model for long-term hepatotoxicity testing utilizing rat hepatocytes entrapped in micro-hollow fiber reactor, Biochemical Engineering Journal, vol.34, issue.3, pp.267-272, 2007.
DOI : 10.1016/j.bej.2006.12.010

C. Legallais, A. Gautier, M. Dufresne, B. Carpentier, and R. Baudoin, The place of adsorption and biochromatography in extracorporeal liver support systems, Journal of Chromatography B, vol.861, issue.2, pp.171-176, 2008.
DOI : 10.1016/j.jchromb.2007.07.048

URL : https://hal.archives-ouvertes.fr/hal-00339871

Q. Meng, G. Zhang, C. Shen, and H. Qiu, Sensitivities of gel entrapped hepatocytes in hollow fibers to hepatotoxic drug, Toxicology Letters, vol.166, issue.1, pp.19-26, 2006.
DOI : 10.1016/j.toxlet.2006.05.008

J. Allen, T. Hassanein, and S. Bhatia, Advances in bioartificial liver devices, Hepatology, vol.34, issue.3, pp.447-455, 2001.
DOI : 10.1053/jhep.2001.26753

R. Gebhardt, J. Hengstler, D. Muller, R. Glockner, P. Buenning et al., New Hepatocyte In Vitro Systems for Drug Metabolism: Metabolic Capacity and Recommendations for Application in Basic Research and Drug Development, Standard Operation Procedures, Drug Metabolism Reviews, vol.258, issue.4, pp.145-213, 2003.
DOI : 10.3109/00498258209046784

Y. Sakai, O. Choi, S. Sakoda, and A. , Development of a biohybrid simulator for absorption and biotransformation processes in humans based on in vitro models of small intestine and liver tissues, Journal of Artificial Organs, vol.6, issue.4, pp.273-281, 2003.
DOI : 10.1007/s10047-003-0238-4

L. Sweeney and M. Shuler, A cell culture analogue of rodent physiology: Application to naphthalene toxicology, Toxicology in Vitro, vol.9, issue.3, pp.307-316, 1995.
DOI : 10.1016/0887-2333(95)00007-U

S. Choi, O. Sakoda, A. Sakai, and Y. , Enhanced cytochrome P450 capacities of Caco-2 and Hep G2 cells in new coculture system under the static and perfused conditions: evidence for possible organ-to-organ interactions against exogenous stimuli, Materials Science and Engineering: C, vol.24, issue.3, pp.333-339, 2004.
DOI : 10.1016/j.msec.2003.12.002

J. El-ali, P. Sorger, and K. Jensen, Cells on chips, Nature, vol.4, issue.7101, pp.403-411, 2006.
DOI : 10.1038/nature05063

P. Abgrall and A. Gue, Lab-on-chip technologies: making a microfluidic network and coupling it into a complete microsystem???a review, Journal of Micromechanics and Microengineering, vol.17, issue.5, pp.15-49, 2007.
DOI : 10.1088/0960-1317/17/5/R01

M. Hu and R. Zengerle, Microfabricated devices for controlled biochemical release, 10th International Conference on New Actuators 14-16 june, pp.263-271, 2006.

E. Meng, J. Shih, P. Li, R. Lo, M. Humayun et al., Electrolysis-driven drug delivery for treatment of ocular disease, The 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS2006) November 5-9 Tokyo Japan, pp.633-635, 2006.

Z. Wang, M. Kim, M. Marquez, and T. Thorsen, High-density microfluidic arrays for cell cytotoxicity analysis, Lab on a Chip, vol.20, issue.88, pp.740-745, 2007.
DOI : 10.1182/blood-2006-08-043570

N. Nguyen and Z. Wu, Micromixers???a review, Journal of Micromechanics and Microengineering, vol.15, issue.2, pp.1-16, 2005.
DOI : 10.1088/0960-1317/15/2/R01

V. Hessel, H. Lowe, and F. Schonfeld, Micromixers???a review on passive and active mixing principles, Chemical Engineering Science, vol.60, issue.8-9, pp.2479-2501, 2005.
DOI : 10.1016/j.ces.2004.11.033

C. Zhang, D. Xing, and Y. Li, Micropumps, microvalves, and micromixers within PCR microfluidic chips: Advances and trends, Biotechnology Advances, vol.25, issue.5, pp.483-514, 2007.
DOI : 10.1016/j.biotechadv.2007.05.003

N. Li, A. Tourovskaia, and A. Folch, Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells, Critical Reviews in Biomedical Engineering, vol.31, issue.5-6, pp.423-488, 2003.
DOI : 10.1615/CritRevBiomedEng.v31.i56.20

G. Walker, H. Zeringue, and D. Beebe, Microenvironment design considerations for cellular scale studies, Lab on a Chip, vol.4, issue.2, pp.91-97, 2004.
DOI : 10.1039/b311214d

E. Verpoorte, Microfluidic chips for clinical and forensic analysis, ELECTROPHORESIS, vol.56, issue.5, pp.677-712, 2002.
DOI : 10.1002/1522-2683(200203)23:5<677::AID-ELPS677>3.0.CO;2-8

D. Weibel and G. Whitesides, Applications of microfluidics in chemical biology, Current Opinion in Chemical Biology, vol.10, issue.6, pp.584-591, 2006.
DOI : 10.1016/j.cbpa.2006.10.016

D. Breslauer, P. Lee, and L. Lee, Microfluidics-based systems biology, Molecular BioSystems, vol.306, issue.2, pp.97-112, 2006.
DOI : 10.1039/b515632g

URL : http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.469.518

J. Allen, S. Khetani, and S. Bhatia, In Vitro Zonation and Toxicity in a Hepatocyte Bioreactor, Toxicological Sciences, vol.84, issue.1, pp.110-119, 2005.
DOI : 10.1093/toxsci/kfi052

L. Kim, Y. Toh, J. Voldman, and H. Yu, A practical guide to microfluidic perfusion culture of adherent mammalian cells, Lab on a Chip, vol.6, issue.6, pp.681-694, 2007.
DOI : 10.1039/b704602b

A. Tilles, H. Baskaran, P. Roy, M. Yarmush, and M. Toner, Effects of oxygenation and flow on the viability and function of rat hepatocytes cocultured in a microchannel flat-plate bioreactor, Biotechnology and Bioengineering, vol.1, issue.5, pp.379-389, 2001.
DOI : 10.1002/bit.1071

V. Bhat, P. Windridge, R. Cherry, and L. Mandel, Fluctuating shear stress effects on stress fiber architecture and energy metabolism of cultured renal cells, Biotechnology Progress, vol.11, issue.5, pp.596-600, 1995.
DOI : 10.1021/bp00035a016

J. Park, F. Berthiaume, M. Toner, M. Yarmush, and A. Tilles, Microfabricated grooved substrates as platforms for bioartificial liver reactors, Biotechnology and Bioengineering, vol.1, issue.5, pp.632-644, 2005.
DOI : 10.1002/bit.20463

R. Flemming, C. Murphy, G. Abrams, S. Goodman, and P. Nealey, Effects of synthetic micro- and nano-structured surfaces on cell behavior, Biomaterials, vol.20, issue.6, pp.573-588, 1999.
DOI : 10.1016/S0142-9612(98)00209-9

S. Sarkar, M. Dadhania, P. Rourke, T. Desai, and J. Wong, Vascular tissue engineering: microtextured scaffold templates to control organization of vascular smooth muscle cells and extracellular matrix, Acta Biomaterialia, vol.1, issue.1, pp.93-100, 2005.
DOI : 10.1016/j.actbio.2004.08.003

E. Leclerc, A. Corlu, L. Griscom, R. Baudoin, and C. Legallais, Guidance of liver and kidney organotypic cultures inside rectangular silicone microchannels, Biomaterials, vol.27, issue.22, pp.4109-4119, 2006.
DOI : 10.1016/j.biomaterials.2006.03.031

URL : https://hal.archives-ouvertes.fr/hal-00090178

A. Grayson, R. Shawgo, A. Johnson, N. Flynn, Y. Li et al., A BioMEMS Review: MEMS Technology for Physiologically Integrated Devices, Proceedings of the IEEE, vol.92, issue.1, pp.6-21, 2004.
DOI : 10.1109/JPROC.2003.820534

D. Duffy, J. Mcdonald, O. Schueller, and G. Whitesides, Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane), Analytical Chemistry, vol.70, issue.23, pp.4974-4984, 1998.
DOI : 10.1021/ac980656z

R. Bashir, BioMEMS: state-of-the-art in detection, opportunities and prospects, Advanced Drug Delivery Reviews, vol.56, issue.11, pp.1565-1586, 2004.
DOI : 10.1016/j.addr.2004.03.002

B. Ziaie, A. Baldi, M. Lei, Y. Gu, and R. Siegel, Hard and soft micromachining for BioMEMS: review of techniques and examples of applications in microfluidics and drug delivery, Advanced Drug Delivery Reviews, vol.56, issue.2, pp.145-172, 2004.
DOI : 10.1016/j.addr.2003.09.001

X. Feng, J. Castracane, N. Tokranova, A. Gracias, G. Lnenicka et al., A living cell-based biosensor utilizing G-protein coupled receptors: Principles and detection methods, Biosensors and Bioelectronics, vol.22, issue.12, pp.3230-3237, 2007.
DOI : 10.1016/j.bios.2007.03.002

E. Leclerc, F. Miyata, K. Furukawa, T. Ushida, Y. Sakai et al., Effect on liver cells of stepwise microstructures fabricated in a photosensitive biodegradable polymer by softlithography, Materials Science and Engineering: C, vol.24, issue.3, pp.349-354, 2004.
DOI : 10.1016/j.msec.2003.12.004

A. Khademhosseini, G. Eng, J. Yeh, J. Fukuda, J. Blumling et al., Micromolding of photocrosslinkable hyaluronic acid for cell encapsulation and entrapment, Journal of Biomedical Materials Research Part A, vol.3, issue.3, pp.522-532, 2006.
DOI : 10.1002/jbm.a.30821

C. Ho, R. Lin, W. Chang, H. Chang, and C. Liu, Rapid heterogeneous liver-cell on-chip patterning via the enhanced field-induced dielectrophoresis trap, Lab on a Chip, vol.6, issue.1, pp.724-734, 2006.
DOI : 10.1039/b602036d

C. Chen, D. Breslauer, J. Luna, A. Grimes, W. Chin et al., Shrinky-Dink microfluidics: 3D polystyrene chips, Lab on a Chip, vol.9, issue.19, pp.622-624, 2008.
DOI : 10.1039/b719029h

S. Takayama, J. Mcdonald, E. Ostuni, M. Liang, P. Kenis et al., Patterning cells and their environments using multiple laminar fluid flows in capillary networks, Proceedings of the National Academy of Sciences, vol.96, issue.10, pp.5545-5548, 1999.
DOI : 10.1073/pnas.96.10.5545

D. Chiu, N. Jeon, S. Huang, R. Kane, C. Wargo et al., Patterned deposition of cells and proteins onto surfaces by using three-dimensional microfluidic systems, Proceedings of the National Academy of Sciences, vol.97, issue.6, pp.2408-2413, 2000.
DOI : 10.1073/pnas.040562297

R. Kane, S. Takayama, E. Ostuni, D. Ingber, and G. Whitesides, Patterning proteins and cells using soft lithography, Biomaterials, vol.20, issue.23-24, pp.2363-2376, 1999.
DOI : 10.1016/S0142-9612(99)00165-9

A. Folch and M. Toner, Microengineering of Cellular Interactions, Annual Review of Biomedical Engineering, vol.2, issue.1, pp.227-256, 2000.
DOI : 10.1146/annurev.bioeng.2.1.227

S. Bhatia, U. Balis, M. Yarmush, and M. Toner, Microfabrication of Hepatocyte/Fibroblast Co-cultures: Role of Homotypic Cell Interactions, Biotechnology Progress, vol.14, issue.3, pp.378-387, 1998.
DOI : 10.1021/bp980036j

Y. Zinchenko and R. Coger, Engineering micropatterned surfaces for the coculture of hepatocytes and Kupffer cells, Journal of Biomedical Materials Research Part A, vol.60, issue.1, pp.242-248, 2005.
DOI : 10.1002/jbm.a.30399

S. Bhatia, U. Balis, M. Yarmush, and M. Toner, Probing heterotypic cell interactions: Hepatocyte function in microfabricated co-cultures, Journal of Biomaterials Science, Polymer Edition, vol.26, issue.11, pp.1137-1160, 1998.
DOI : 10.1163/156856298X00695

M. Kim, J. Yeon, and J. Park, A microfluidic platform for 3-dimensional cell culture and cell-based assays, Biomedical Microdevices, vol.16, issue.1, pp.25-34, 2007.
DOI : 10.1007/s10544-006-9016-4

W. Koh and M. Pishko, Fabrication of cell-containing hydrogel microstructures inside microfluidic devices that can be used as cell-based biosensors, Analytical and Bioanalytical Chemistry, vol.34, issue.8, pp.1389-1397, 2006.
DOI : 10.1007/s00216-006-0571-6

R. Gunawan, E. Choban, J. Conour, J. Silvestre, L. Schook et al., Regiospecific Control of Protein Expression in Cells Cultured on Two-Component Counter Gradients of Extracellular Matrix Proteins, Langmuir, vol.21, issue.7, pp.3061-3068, 2005.
DOI : 10.1021/la048303k

S. Rhee, A. Taylor, D. Cribbs, C. Cotman, and N. Jeon, External force-assisted cell positioning inside microfluidic devices, Biomedical Microdevices, vol.92, issue.1, pp.15-23, 2007.
DOI : 10.1007/s10544-006-9002-x

E. Leclerc, E. Kirat, K. Griscom, and L. , In situ micropatterning technique by cell crushing for co-cultures inside microfluidic biochips, Biomedical Microdevices, vol.13, issue.1, pp.169-177, 2007.
DOI : 10.1007/s10544-007-9122-y

URL : https://hal.archives-ouvertes.fr/hal-00308481

P. Lee, P. Hung, and L. Lee, An artificial liver sinusoid with a microfluidic endothelial-like barrier for primary hepatocyte culture, Biotechnology and Bioengineering, vol.27, issue.5, pp.1340-1346, 2007.
DOI : 10.1002/bit.21360

Y. Toh, C. Zhang, J. Zhang, Y. Khong, S. Chang et al., A novel 3D mammalian cell perfusion-culture system in microfluidic channels, Lab on a Chip, vol.113, issue.4, pp.302-309, 2007.
DOI : 10.1039/b614872g

G. Underhill, A. Chen, D. Albrecht, and S. Bhatia, Assessment of hepatocellular function within PEG hydrogels, Biomaterials, vol.28, issue.2, pp.256-270, 2007.
DOI : 10.1016/j.biomaterials.2006.08.043

A. Revzin, R. Russell, V. Yadavalli, W. Koh, C. Deister et al., Fabrication of Poly(ethylene glycol) Hydrogel Microstructures Using Photolithography, Langmuir, vol.17, issue.18, pp.5440-5447, 2001.
DOI : 10.1021/la010075w

V. Tsang and S. Bhatia, Fabrication of Three-Dimensional Tissues, Adv Drug Deliv Rev, vol.56, pp.1635-1647, 2004.
DOI : 10.1007/10_010

W. Koh, L. Itle, and M. Pishko, Molding of Hydrogel Microstructures to Create Multiphenotype Cell Microarrays, Analytical Chemistry, vol.75, issue.21, pp.5783-5789, 2003.
DOI : 10.1021/ac034773s

D. Kim, W. Lee, and W. Koh, Micropatterning of proteins on the surface of three-dimensional poly(ethylene glycol) hydrogel microstructures, Analytica Chimica Acta, vol.609, issue.1, pp.59-65, 2008.
DOI : 10.1016/j.aca.2007.12.024

F. Wang, H. Wang, J. Wang, H. Wang, P. Rummel et al., Microfluidic delivery of small molecules into mammalian cells based on hydrodynamic focusing, Biotechnology and Bioengineering, vol.5, issue.1, pp.150-158, 2008.
DOI : 10.1002/bit.21737

K. Wada, A. Taniguchi, J. Kobayashi, M. Yamato, and T. Okano, Live cells-based cytotoxic sensorchip fabricated in a microfluidic system, Biotechnology and Bioengineering, vol.1, issue.6, pp.1513-71513, 2008.
DOI : 10.1002/bit.21718

C. Luo, X. Zhu, T. Yu, X. Luo, Q. Ouyang et al., A fast cell loading and high-throughput microfluidic system for long-term cell culture in zero-flow environments, Biotechnology and Bioengineering, vol.79, issue.1, pp.190-195, 2008.
DOI : 10.1002/bit.21877

N. Ye, J. Qin, W. Shi, X. Liu, and B. Lin, Cell-based high content screening using an integrated microfluidic device, Lab on a Chip, vol.392, issue.12, pp.1696-1704, 2007.
DOI : 10.1039/b711513j

K. King, S. Wang, D. Irimia, A. Jayaraman, M. Toner et al., A high-throughput microfluidic real-time gene expression living cell array, Lab Chip, vol.13, issue.1, pp.77-85, 2007.
DOI : 10.1039/B612516F

P. Hung, P. Lee, P. Sabounchi, N. Aghdam, R. Lin et al., A novel high aspect ratio microfluidic design to provide a stable and uniform microenvironment for cell growth in a high throughput mammalian cell culture array, Lab on a Chip, vol.5, issue.1, pp.44-48, 2005.
DOI : 10.1039/b410743h

S. Sugiura, J. Edahiro, K. Kikuchi, K. Sumaru, and T. Kanamori, Pressure-driven perfusion culture microchamber array for a parallel drug cytotoxicity assay, Biotechnology and Bioengineering, vol.59, issue.6, 2008.
DOI : 10.1002/bit.21836

J. Yoon, J. Choi, I. Lee, and M. Kim, A valveless micropump for bidirectional applications, Sensors and Actuators A: Physical, vol.135, issue.2, pp.833-838, 2007.
DOI : 10.1016/j.sna.2006.08.017

K. Oh and C. Ahn, A review of microvalves, Journal of Micromechanics and Microengineering, vol.16, issue.5, pp.13-39, 2006.
DOI : 10.1088/0960-1317/16/5/R01

C. Yi, C. Li, J. S. Yang, and M. , Microfluidics technology for manipulation and analysis of biological cells, Analytica Chimica Acta, vol.560, issue.1-2, pp.1-23, 2006.
DOI : 10.1016/j.aca.2005.12.037

K. Viravaidya, A. Sin, and M. Shuler, Development of a Microscale Cell Culture Analog To Probe Naphthalene Toxicity, Biotechnology Progress, vol.53, issue.4, pp.316-323, 2004.
DOI : 10.1021/bp0341996

K. Viravaidya and M. Shuler, Incorporation of 3T3-L1 Cells To Mimic Bioaccumulation in a Microscale Cell Culture Analog Device for Toxicity Studies, Biotechnology Progress, vol.49, issue.6, pp.590-597, 2004.
DOI : 10.1021/bp034238d

R. Baudoin, L. Griscom, M. Monge, C. Legallais, and E. Leclerc, Development of a Renal Microchip for In Vitro Distal Tubule Models, Biotechnology Progress, vol.0, issue.0, pp.1245-1253, 2007.
DOI : 10.1021/bp0603513

URL : https://hal.archives-ouvertes.fr/hal-00196149

E. Leclerc, Y. Sakai, and T. Fujii, Cell culture in 3-Dimensional microfluidic structure of pdms (polydimethylsiloxane), Biomedical Microdevices, vol.5, issue.2, pp.109-114, 2003.
DOI : 10.1023/A:1024583026925

E. Leclerc, Y. Sakai, and T. Fujii, Microfluidic PDMS (Polydimethylsiloxane) Bioreactor for Large-Scale Culture of Hepatocytes, Biotechnology Progress, vol.20, issue.3, pp.750-755, 2004.
DOI : 10.1021/bp0300568

K. Domansky, A. Sivaraman, and L. Griffith, Micromachined Bioreactor for in Vitro Cell Self- Assembly and 3D Tissue Formation. Lab-on-chips for cellomics, pp.319-346, 2004.

M. Powers, K. Domansky, M. Kaazempur-mofrad, A. Kalezi, A. Capitano et al., A microfabricated array bioreactor for perfused 3D liver culture, Biotechnology and Bioengineering, vol.156, issue.3, pp.257-269, 2002.
DOI : 10.1002/bit.10143

J. Park, Y. Li, F. Berthiaume, M. Toner, M. Yarmush et al., Radial flow hepatocyte bioreactor using stacked microfabricated grooved substrates, Biotechnology and Bioengineering, vol.1, issue.5, pp.455-467, 2008.
DOI : 10.1002/bit.21572

E. Leclerc, B. David, L. Griscom, B. Lepioufle, T. Fujii et al., Study of osteoblastic cells in a microfluidic environment, Biomaterials, vol.27, issue.4, pp.586-595, 2006.
DOI : 10.1016/j.biomaterials.2005.06.002

URL : https://hal.archives-ouvertes.fr/inserm-00166082

E. Leclerc, Y. Sakai, and T. Fujii, Perfusion culture of fetal human hepatocytes in microfluidic environments, Biochemical Engineering Journal, vol.20, issue.2-3, pp.143-148, 2004.
DOI : 10.1016/j.bej.2003.09.010

M. Zhang, P. Lee, P. Hung, T. Johnson, L. Lee et al., Microfluidic environment for high density hepatocyte culture, Biomedical Microdevices, vol.169, issue.1, pp.117-121, 2008.
DOI : 10.1007/s10544-007-9116-9

J. Anderson, D. Chiu, R. Jackman, O. Cherniavskaya, J. Mcdonald et al., Fabrication of Topologically Complex Three-Dimensional Microfluidic Systems in PDMS by Rapid Prototyping, Analytical Chemistry, vol.72, issue.14, pp.3158-3164, 2000.
DOI : 10.1021/ac9912294

R. Jackman, J. Wilbur, and G. Whitesides, Fabrication of submicrometer features on curved substrates by microcontact printing, Science, vol.269, issue.5224, pp.664-666, 1995.
DOI : 10.1126/science.7624795

V. Linder, H. Wu, X. Jiang, and G. Whitesides, Rapid Prototyping of 2D Structures with Feature Sizes Larger than 8 ??m, Analytical Chemistry, vol.75, issue.10, pp.2522-2527, 2003.
DOI : 10.1021/ac026441d

S. Li and S. Chen, Polydimethylsioxane Fluidic Interconnects for Microfluidic Systems, IEEE Transactions on advanced Packaging, vol.26, pp.242-247, 2003.

F. Briquet, A. Colas, and X. Thomas, Silicones à usage médical Dow Corning France -Division Santé, Europe, pp.1-11, 1996.

C. Filippi, S. Keatch, D. Rangar, L. Nelson, P. Hayes et al., Improvement of C3A cell metabolism for usage in bioartificial liver support systems, Journal of Hepatology, vol.41, issue.4, pp.599-605, 2004.
DOI : 10.1016/j.jhep.2004.06.012

T. Elkayam, S. Mitay-shaprut, M. Dvir-ginzberg, T. Harel, and S. Cohen, Enhancing the Drug Metabolism Activities of C3A??? A Human Hepatocyte Cell Line???By Tissue Engineering Within Alginate Scaffolds, Tissue Engineering, vol.12, issue.5, pp.1357-1368, 2006.
DOI : 10.1089/ten.2006.12.1357

N. Krasteva, B. Seifert, W. Albrecht, T. Weigel, M. Schossig et al., Influence of polymer membrane porosity on C3A hepatoblastoma cell adhesive interaction and function, Biomaterials, vol.25, issue.13, pp.2467-2476, 2004.
DOI : 10.1016/j.biomaterials.2003.09.031

M. Schneider, I. Marison, and V. Su, The importance of ammonia in mammalian cell culture, Journal of Biotechnology, vol.46, issue.3, pp.161-185, 1996.
DOI : 10.1016/0168-1656(95)00196-4

C. Kemp and J. Shiloach, A simple colorimetric assay for the determination of L-glutamine in samples of tissue culture medium, Am Biotechnol Lab, vol.11, p.12, 1993.

M. Burke and R. Mayer, Ethoxyresorufin: direct fluorimetric assay of a microsomal Odealkylation which is preferentially inducible by 3-methylcholanthrene, Drug Metab Dispos, vol.2, pp.583-588, 1974.

J. Kelly and N. Sussman, A Fluorescent Cell-Based Assay for Cytochrome P-450 Isozyme 1A2 Induction and Inhibition, Journal of Biomolecular Screening, vol.87, issue.4, pp.249-253, 2000.
DOI : 10.1177/108705710000500407

S. Siegel, C. Jr, and N. , Nonparametric statistics for the behavioral sciences, Social Sciences/Languages, 1988.

J. Georgin and M. Gouet, Statistiques avec Excel, 1ère édition, vol.Eyrolles, p.352, 2000.

D. Mavri-damelin, L. Damelin, S. Eaton, M. Rees, C. Selden et al., Cells for bioartificial liver devices: The human hepatoma-derived cell line C3A produces urea but does not detoxify ammonia, Biotechnology and Bioengineering, vol.336, issue.3, pp.644-651, 2008.
DOI : 10.1002/bit.21599

S. Gillmor, B. Larson, J. Braun, C. Mason, L. Cruz-barba et al., Lowcontact-angle polydimethyl siloxane (PDMS) membranes for fabricating microbioarrays, Microtechnologies in Medicine & Biology, pp.51-56, 2002.
DOI : 10.1109/mmb.2002.1002263

D. Eddington, J. Puccinelli, and D. Beebe, Thermal aging and reduced hydrophobic recovery of polydimethylsiloxane, Sensors and Actuators B: Chemical, vol.114, issue.1, pp.170-172, 2006.
DOI : 10.1016/j.snb.2005.04.037

A. Sanchez, A. Alvarez, R. Pagan, C. Roncero, S. Vilaro et al., Fibronectin regulates morphology, cell organization and gene expression of rat fetal hepatocytes in primary culture, Journal of Hepatology, vol.32, issue.2, pp.242-250, 2000.
DOI : 10.1016/S0168-8278(00)80069-0

C. Trautwein, M. Davies, E. Elias, A. Strain, and M. Manns, Extracellular matrix proteins modulate cytochrome P450 2D6 expression in human hepatocytes, Journal of Hepatology, vol.22, issue.1, pp.50-56, 1995.
DOI : 10.1016/0168-8278(95)80259-2

S. Jiang, W. Chuang, and M. Tang, Role of fibronectin deposition in branching morphogenesis of Madin-Darby canine kidney cells, Kidney International, vol.57, issue.5, pp.1860-71860, 2000.
DOI : 10.1046/j.1523-1755.2000.00035.x

A. Masumoto, S. Arao, and M. Otsuki, Role of ?1 integrins in adhesion and invasion of hepatocellular carcinoma cells, Hepatology, vol.17, issue.1, pp.68-74, 1999.
DOI : 10.1002/hep.510290146

C. Schoenenberger, A. Zuk, G. Zinkl, D. Kendall, and K. Matlin, Integrin expression and localization in normal MDCK cells and transformed MDCK cells lacking apical polarity, J Cell Sci, vol.107, issue.2, pp.527-541, 1994.

B. Keselowsky, D. Collard, and A. Garcia, Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion, Journal of Biomedical Materials Research Part A, vol.60, issue.Pt 3, pp.247-259, 2003.
DOI : 10.1002/jbm.a.10537

E. Leclerc, K. Furukawa, F. Miyata, Y. Sakai, T. Ushida et al., Fabrication of microstructures in photosensitive biodegradable polymers for tissue engineering applications, Biomaterials, vol.25, issue.19, pp.4683-4690, 2004.
DOI : 10.1016/j.biomaterials.2003.10.060

J. Fukuda, A. Khademhosseini, Y. Yeo, X. Yang, J. Yeh et al., Micromolding of photocrosslinkable chitosan hydrogel for spheroid microarray and co-cultures, Biomaterials, vol.27, issue.30, pp.5259-5267, 2006.
DOI : 10.1016/j.biomaterials.2006.05.044

S. Nyberg, J. Hardin, B. Amiot, U. Argikar, R. Remmel et al., Rapid, large-scale formation of porcine hepatocyte spheroids in a novel spheroid reservoir bioartificial liver, Liver Transplantation, vol.29, issue.8, pp.901-910, 2005.
DOI : 10.1002/lt.20446

H. Yu, I. Meyvantsson, I. Shkel, and D. Beebe, Diffusion dependent cell behavior in microenvironments, Lab on a Chip, vol.113, issue.4, pp.1089-1095, 2005.
DOI : 10.1039/b504403k

T. Torii, M. Miyazawa, and I. Koyama, Effect of Continuous Application of Shear Stress on Liver Tissue: Continuous Application of Appropriate Shear Stress Has Advantage in Protection of Liver Tissue, Transplantation Proceedings, vol.37, issue.10, pp.4575-4578, 2005.
DOI : 10.1016/j.transproceed.2005.10.118

H. Nakatsuka, T. Sokabe, K. Yamamoto, Y. Sato, K. Hatakeyama et al., Shear stress induces hepatocyte PAI-1 gene expression through cooperative Sp1/Ets-1 activation of transcription, AJP: Gastrointestinal and Liver Physiology, vol.291, issue.1, pp.26-34, 2006.
DOI : 10.1152/ajpgi.00467.2005

M. Jensen, E. Odgaard, M. Christensen, H. Praetorius, and J. Leipziger, Flow-Induced [Ca2+]i Increase Depends on Nucleotide Release and Subsequent Purinergic Signaling in the Intact Nephron, Journal of the American Society of Nephrology, vol.18, issue.7, pp.2062-2070, 2007.
DOI : 10.1681/ASN.2006070700

H. Praetorius, J. Frokiaer, and J. Leipziger, Transepithelial pressure pulses induce nucleotide release in polarized MDCK cells, AJP: Renal Physiology, vol.288, issue.1, pp.133-141, 2005.
DOI : 10.1152/ajprenal.00238.2004

A. Rotem, M. Toner, R. Tompkins, and M. Yarmush, Oxygen uptake rates in cultured rat hepatocytes, Biotechnology and Bioengineering, vol.65, issue.10, pp.1286-1291, 1992.
DOI : 10.1002/bit.260401020

U. Balis, K. Behnia, B. Dwarakanath, S. Bhatia, S. Sullivan et al., Oxygen Consumption Characteristics of Porcine Hepatocytes, Metabolic Engineering, vol.1, issue.1, pp.49-62, 1999.
DOI : 10.1006/mben.1998.0105

A. Guillouzo, C. Guyomard, A. Fautrel, and C. Chesné, Storage of isolated hepatocytes. The hepatocyte review, pp.125-145, 2000.

M. Bayliss and D. Cross, The importance of hepatocytes in drug metabolism studies : an industrial perspective. The hepatocyte review, pp.365-389, 2000.

S. Schmitmeier, A. Langsch, and A. Bader, A membrane-based small-scale bioreactor for accelerated in vitro drug screenings with primary hepatocytes, Desalination, vol.199, issue.1-3, pp.258-260, 2006.
DOI : 10.1016/j.desal.2006.03.062

E. Alexandre, P. David, C. Viollon, P. Wolf, D. Jaeck et al., Expression of Cytochromes P-450 2E1, 3A4 and 1A1/1A2 in Growing and Confluent Human HepG2 Hepatoma Cells???Effect of Ethanol, Toxicology in Vitro, vol.13, issue.3, pp.427-435, 1999.
DOI : 10.1016/S0887-2333(99)00007-7

I. Schaeffner, J. Petters, H. Aurich, P. Frohberg, and C. B. , A Microtiterplate-Based Screening Assay to Assess Diverse Effects on Cytochrome P450 Enzyme Activities in Primary Rat Hepatocytes by Various Compounds, ASSAY and Drug Development Technologies, vol.3, issue.1, pp.27-38, 2005.
DOI : 10.1089/adt.2005.3.27