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Développement et caractérisation d'une puce à cellules pour le criblage d'agents toxiques

Abstract : Current developments in tissue engineering and microtechnology fields allowed proposing new pertinent tools to investigate in vitro toxicity.
We propose the development of a cellular microchip that mimics organs in vitro. To validate our approach, we showed the pertinence of the microchip environment with the culture of a renal cell line, the Madin Darby Canine Kidney (MDCK) and a human hepatic cell line, the HepG2/C3A. In this study, we tested three flow rates (0, 10 and 25 µL/min) at three inoculated cell densities. Then, we tested our microchip with three ammonium chloride loadings (0, 5 and 10mM) in order to demonstrate its potential for future toxicity experiments. The cellular activities were monitored by the cell proliferation rates, the glucose and glutamine consumptions, albumin and ammonia productions and by the detoxication via the CYP 1A activity.
In dynamic condition, the cellular activities in term of consumptions and productions were higher in the microchip than in static conditions. More especially, the detoxication activity of the CYP 1A was found higher. The toxicity analysis with the chloride ammonium showed similar tendencies in the microchip and in Petri standard culture conditions (reduction of proliferation. However, the ammonium chloride seemed induce a higher CYP 1A activity in the microchip.
By these investigations, we showed the pertinence of the utilization of our microchip for in vitro dynamic toxicity testing. The targeted industries of this new in vitro cell culture model are the chemical, pharmaceutical and cosmetic industries.
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Submitted on : Thursday, November 27, 2008 - 11:17:28 AM
Last modification on : Monday, September 3, 2018 - 1:33:05 PM
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R. Baudoin. Développement et caractérisation d'une puce à cellules pour le criblage d'agents toxiques. Sciences de l'ingénieur [physics]. Université de Technologie de Compiègne, 2008. Français. ⟨tel-00342342⟩

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