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PHASES DENSES DE COLLAGÈNE DE TYPE I :
TRANSITION ISOTROPE/CHOLESTÉRIQUE, FIBRILLOGENÈSE ET MINÉRALISATION

Abstract : The aim of this work is the in vitro study the self-assembly properties of collagen molecules in dense phases. First, we focused on acidic colloidal suspensions of collagen. We studied the isotropic/cholesteric (I/N*) transition as well as the structure of the liquid-crystalline phase thus obtained. For this we used polarized light optical microscopy and small angle X-ray scattering. Moreover, we described the rheological properties of these solutions through oscillating experiments with a cone-plane rheometer. Then we studied the influence of simple physico-chemical parameters on the formation of fibrillar gels resulting from the neutralization of acidic solution (« fibrillogenesis »). These gels were characterized by X-ray scattering and Transmission Electron Microscopy on ultrathin sections. Finally, we present some results concerning the mineralization of such ordered fibrillar matrices ; in particular, we highlight the coalignment of a hydroxyapatite phase along with the organic one. This work takes place in a wider context of tissue morphogenesis comprehension and of ordered biomaterials synthesis.
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https://tel.archives-ouvertes.fr/tel-00337402
Contributor : Frédéric Gobeaux Connect in order to contact the contributor
Submitted on : Thursday, November 6, 2008 - 7:42:58 PM
Last modification on : Thursday, November 18, 2021 - 4:06:25 AM
Long-term archiving on: : Saturday, November 26, 2016 - 2:29:48 AM

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  • HAL Id : tel-00337402, version 1

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Frédéric Gobeaux. PHASES DENSES DE COLLAGÈNE DE TYPE I :
TRANSITION ISOTROPE/CHOLESTÉRIQUE, FIBRILLOGENÈSE ET MINÉRALISATION. Biophysique [physics.bio-ph]. Université Pierre et Marie Curie - Paris VI, 2007. Français. ⟨tel-00337402⟩

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