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DYNAMICS OF CELL WALL PROTEINS DURING CELL ELONGATION IN ETIOLATED HYPOCOTYLS OF ARABIDOPSIS THALIANA AS SHOWN BY PROTEOMIC AND TRANSCRIPTOMIC SURVEYS

Abstract : The cell wall of higher plants is a complex dynamic entity that performs a variety of functions during growth and development as well as in response to environmental stresses. Cell wall proteins play important roles in cell elongation. Etiolated hypocotyls of Arabidopsis thaliana were chosen as elongating organs since they undergo rapid and polar elongation without cell division. Two developmental stages were compared through proteomic and transcriptomic surveys: active elongation (5- day-old hypocotyls) vs after growth arrest (11 day-old hypocotyls). As a prerequisite to proteomic study, an efficient cell wall purification method was established as well as a powerful strategy for protein separation. Proteins were identified by peptide mass mapping using MALDI-TOF mass spectrometry and bioinformatics. This study resulted in identification of 137 proteins among which 51 were not identified before by proteomics, and 36 (resp. 17) were found only at 5-days (resp. 11-days). Overall, many protein families expected to be involved in cell wall elongation or in elongation arrest were found by either survey such as XTHs, PGs, expansins, peroxidases, and laccases. However, some of them were found all along hypocotyl development which suggests other roles in cell walls. In addition, many proteins not described before to play roles during cell wall elongation were identified, such as proteases, proteins related to lipid metabolism and proteins of yet unknown function. Finally, proteomic and transcriptomic results revealed no systematic consistency between them suggesting post-transcriptional regulation steps for genes encoding cell wall proteins.
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https://tel.archives-ouvertes.fr/tel-00323217
Contributor : Rafael F. Pont-Lezica <>
Submitted on : Friday, September 19, 2008 - 5:08:15 PM
Last modification on : Friday, January 10, 2020 - 9:08:46 PM
Long-term archiving on: : Saturday, November 26, 2016 - 12:59:19 AM

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  • HAL Id : tel-00323217, version 1

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Muhammad Irshad. DYNAMICS OF CELL WALL PROTEINS DURING CELL ELONGATION IN ETIOLATED HYPOCOTYLS OF ARABIDOPSIS THALIANA AS SHOWN BY PROTEOMIC AND TRANSCRIPTOMIC SURVEYS. Vegetal Biology. Université Paul Sabatier - Toulouse III, 2008. English. ⟨tel-00323217⟩

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