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AGREGATION DE PROTEINES DE SOIE DANS UN ENVIRONNEMENT MICROFLUIDIQUE

Abstract : Silk is a biopolymer spun by several arthropods. This fibre is made of protein arranged in a semi-crystalline microstructure and has remarkable mechanical properties. The main axis of this thesis work concerns the comprehension of silk fibre formation. Bombyx mori silk has been used as a model. The protein component of B. mori silk, named Fibroin, has been spun into a fibre in a microfluidic device designed to mimic the silkworm spinning apparatus. The silk formation process has been followed by synchrotron radiation scattering (SAXS and WAXS) and Raman spectroscopy. It starts with an aggregation phase. The size of aggregates is around 100 nm. Inside these particles, the Fibroin is compacted.This aggregation is followed by a phase of compaction of the aggregates. Later, within a few hours, the Fibroin undergoes a conformational transition from a mainly amorphous structure (Silk I) to the semi-crystalline structure characteristic of the natural fibre (Silk II). This process is proposed as a possible model of silk formation in vivo. The second axis of this work is dedicated to the investigation of the physical properties of natural B. mori silk. Its resistance to high temperature is studied from a molecular, structural and mechanical point of view. The semi-crystalline structure of the fibre remains stable up to carbonization although its mechanical properties and the Fibroin's integrity are affected at lower temperature. The effect of high pressure on the fibre structure is also presented : throughout the pressure increase, the crystallites can compact slightly before their disorganization.
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https://tel.archives-ouvertes.fr/tel-00322259
Contributor : Anne Martel <>
Submitted on : Wednesday, September 17, 2008 - 10:07:08 AM
Last modification on : Wednesday, September 17, 2008 - 10:16:54 AM
Long-term archiving on: : Thursday, June 3, 2010 - 7:19:03 PM

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  • HAL Id : tel-00322259, version 1

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Anne Martel. AGREGATION DE PROTEINES DE SOIE DANS UN ENVIRONNEMENT MICROFLUIDIQUE. Data Analysis, Statistics and Probability [physics.data-an]. Université Joseph-Fourier - Grenoble I, 2008. English. ⟨tel-00322259⟩

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