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Origin, characterization and roles of matrix vesicles in physiological and pathological mineralization.

Abstract : Matrix vesicles (MVs) are involved in the initiation of mineralization in tissues undergoing physiological and pathological calcification. Pyrophosphate (PPi) has a dual effect on mineralization: a source of phosphate (Pi) to sustain hydroxyapatite (HA) formation and an inhibitor of HA growth. We found that formation of HA was optimal when the Pi/PPi molar ratio was above 140, while calcium pyrophosphate dihydrate, identified in osteoarthritis was exclusively produced by MVs when the ratio was below 6. Proteomic analysis and lipid compositions on both MVs and microvilli from osteoblast-like Saos-2 cells, revealed that MVs have an endoplasmic reticular origin and characteristic lipids and proteins as in lipid rafts. Finally, we demonstrated that MV release from microvilli is caused by the concomitant actions of actin-depolymerizing and contractile motor proteins. Proteins involved in MV biogenesis could be new therapeutic targets to prevent pathological calcification.
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https://tel.archives-ouvertes.fr/tel-00304214
Contributor : Cyril Thouverey <>
Submitted on : Tuesday, July 22, 2008 - 4:11:08 PM
Last modification on : Monday, November 23, 2020 - 12:52:03 PM
Long-term archiving on: : Monday, May 31, 2010 - 8:28:18 PM

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  • HAL Id : tel-00304214, version 1

Citation

Cyril Thouverey. Origin, characterization and roles of matrix vesicles in physiological and pathological mineralization.. Life Sciences [q-bio]. Université Claude Bernard - Lyon I, 2008. English. ⟨tel-00304214⟩

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