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Étude d?un complexe de trois protéines centrales de la division du pneumocoque: DivIB, DivIC et FtsL. Cible thérapeutique?

Audrey Le Gouellec 1
1 LIM - Laboratoire d'Ingénierie des Macromolécules
IBS - UMR 5075 - Institut de biologie structurale
Abstract : S. pneumoniae causes more than one million deaths per year worlwide. Understanding cell division, a vital process for bacteria, is important in order to find innovative therapeutic molecules. DivIB/FtsQ, DivIC/FtsB and FtsL are central proteins of the divisome assembly to the septum. We have demonstrated that they form an essential ternary complex for pneumococcal division. Contrary to both ftsL and divIC, divIB is not essential for viability and growth of S. pneumoniae in rich medium. However, the divIB null mutant does not grow in chemically defined medium. In the absence of DivIB and protein synthesis, the partner FtsL is rapidly degraded, whereas other division proteins are not affected, pointing to a role of DivIB in stabilizing FtsL. This is further supported by the finding that an additional copy of ftsL restores growth of the divIB null mutant in defined medium. Functional mapping of the three distinct a, ß and gamma-domains of the extracellular region of DivIB revealed that a complete ß domain is required to fully rescue the deletion mutant. Most importantly, the deletion of divIB increases the susceptibility to ß-lactams, more evidently in resistant strain, suggesting a function in cell wall synthesis. DivIB would be a good target to restore the efficiency of the most used antibiotics in the world, the ß-lactams.
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https://tel.archives-ouvertes.fr/tel-00303328
Contributor : Audrey Le Gouellec <>
Submitted on : Monday, July 21, 2008 - 9:25:43 PM
Last modification on : Wednesday, November 18, 2020 - 3:36:02 PM
Long-term archiving on: : Monday, May 31, 2010 - 8:42:28 PM

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Audrey Le Gouellec. Étude d?un complexe de trois protéines centrales de la division du pneumocoque: DivIB, DivIC et FtsL. Cible thérapeutique?. Sciences du Vivant [q-bio]. Université Joseph-Fourier - Grenoble I, 2008. Français. ⟨tel-00303328⟩

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