Skip to Main content Skip to Navigation
Theses

Étude de complexes ribonucléoprotéiques impliqués dans la régulation de l'expression des protéines au cours de l'initiation de la traduction

Abstract : Gene expression is frequently regulated during cellular life. Protein synthesis, also called translation, is the last part of that expression and is wholly regulated. Translation is divided in three parts: initiation, elongation and termination. Initiation commences by the 40S ribosomal subunit binding on the mRNA then scanned by her until it reaches the initiation codon. Many initiation factors are involved. Among them, eIF3 directly bind that subunit. During my work, I studied in a first part, the putative interaction between the factor eIF3 and the small ribosomal subunit 40S. The ribosome is constituted of two different entities: ribosomal proteins and rRNA. The factor eIF3, contains at least 13 subunits, two possess a RRM, and are potentially able to bind that rRNA via their RRM: p44 et p116. eIF3p44 showed previously that she was able to bind 18S rRNA. I screened a library of this rRNA fragments in the aim of identifying a binding site of p44 on the 40S subunit. The new synthesized RNAs are transported and localized to allow right spatially and temporally expression. In a second part, I studied interactions involved in translation initiation control of a S. cerevisiae localized mRNA, ASH1, during its transport. Its regulation is mediated through its direct interaction with Khd1p, a three KH domain protein, on one of its RNA localization elements. This interaction is abolished by Khd1p phosphorylation when ASH1 mRNA is well localized.
Complete list of metadatas

https://tel.archives-ouvertes.fr/tel-00278756
Contributor : Marie Ménade <>
Submitted on : Tuesday, May 13, 2008 - 10:17:18 PM
Last modification on : Friday, November 6, 2020 - 4:10:25 AM
Long-term archiving on: : Friday, May 28, 2010 - 7:09:54 PM

Identifiers

  • HAL Id : tel-00278756, version 1

Collections

CNRS | UJF | UGA

Citation

Marie Ménade. Étude de complexes ribonucléoprotéiques impliqués dans la régulation de l'expression des protéines au cours de l'initiation de la traduction. Biochimie [q-bio.BM]. Université Joseph-Fourier - Grenoble I, 2007. Français. ⟨tel-00278756⟩

Share

Metrics

Record views

208

Files downloads

588