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Maintenance génomique chez l'Archaea hyperthermophile Pyrococcus abyssi : découverte de nouvelles interactions physiques et caractérisation fonctionnelle

Abstract : Archaea are micro-organisms present in all ecosystems, but are majoritary present in extremophilic environments. Hyperthermophilic Archaea, as Pyrococcus abyssi, are permanently exposed at temperature which can increase the rate of spontaneous DNA damage. It is probable that hyperthermophilic Archaea possess efficient molecular mechanism to duplicate, maintain and stabilize their genomes. The aim of this project was to investigate the interaction network involved in the process of DNA replication and DNA repair. The methodological approach consists in the coupling of pull-down with mass spectrometry identification of interacting partners. A preliminary interaction network was identified that was composed of new proteins as well as unsuspected interactions between known components of DNA replication machinery. The main results consist in: (1) the nuclease Pab2263, which interacts with PCNA, is a novel player in DNA metabolism. (2) PCNA is likely part of a macrocomplex with the ubiquitary proteins Mre11 and Rad50 suggesting a role of this complex in the repair of double strand break in connection with a replication fork. (3) Fen1 and DNA primase interact physically and can collaborate in vitro to resolve intermediate step of the base excision repair pathway. These results enhance our understanding of DNA repair process in Archaea.
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https://tel.archives-ouvertes.fr/tel-00273692
Contributor : Julien Briffotaux <>
Submitted on : Tuesday, April 15, 2008 - 10:42:33 PM
Last modification on : Wednesday, September 16, 2020 - 9:56:40 AM
Long-term archiving on: : Thursday, May 20, 2010 - 11:22:47 PM

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  • HAL Id : tel-00273692, version 1

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Julien Briffotaux. Maintenance génomique chez l'Archaea hyperthermophile Pyrococcus abyssi : découverte de nouvelles interactions physiques et caractérisation fonctionnelle. Biochimie [q-bio.BM]. Université de Bretagne occidentale - Brest, 2008. Français. ⟨tel-00273692⟩

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