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Mise au point d'une méthode de fonctionnalisation de microcomposants par vois photochimique.

Abstract : The aim of our work is to develop an effective method for the patterning of closed solid support such as microchannels or capillaries by biomolecules. The covalent attachment of biomolecules on the glass and silicon surfaces is carried out by using the formation an oxime linkage. Patterning is achieved upon illumination by deprotecting the aminooxy function protected by a photolabile (NPPOC) group. We have show that on both planar format and capillary format the aminooxy surface protected by a photolabile group, is effective for the immobilization of several oligonucleotides at predefined positions. The strategy was further extended for the immobilization of other molecules such as sugars, peptides or different hydrophobic and hydrophilic molecules in the capillaries. The results show the effectiveness of our strategy for the immobilization of variety of molecules. Further, we showed that the use of a hydrophilic surface (PEG) improves the sensitivity of this methodology.
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https://tel.archives-ouvertes.fr/tel-00272906
Contributor : Nabil Dendane <>
Submitted on : Sunday, April 13, 2008 - 2:51:22 PM
Last modification on : Friday, November 6, 2020 - 4:02:36 AM
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  • HAL Id : tel-00272906, version 1

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Nabil Dendane. Mise au point d'une méthode de fonctionnalisation de microcomposants par vois photochimique.. Chimie. Université Joseph-Fourier - Grenoble I, 2007. Français. ⟨tel-00272906⟩

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