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ETUDE STRUCTURALE ET FONCTIONNELLE DE LA PROTEINE CHAPERON D'HISTONES ASF1

Abstract : Eukaryotic genomic DNA is packed into chromatine as nucleosomes. This packing is essential for the DNA to fit inside the nucleus and for protecting DNA against oxydative aggressions. Additionally, the chromatine structure tightly regulates processes occurring on the DNA template. This work is dedicated to understanding the molecular mechanism underlying chromatin remodeling. Here we focus on the histones H3 and H4, two of the four nucleosome core particules, and the histone chaperone Asf1. We solved by NMR the structure of Asf1 in complex with the histones. This result constitute an essential step firstly to study in vivo the function of this interaction and secondly to initiate the design of specific peptidic inhibitors of this interaction which are also potential anticancer agents. We also developped an in vitro method to study the molecular basis of nucleosome assembly and disasssembly by Asf1.
Keywords : ASF1
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https://tel.archives-ouvertes.fr/tel-00268886
Contributor : Magali Le Discorde <>
Submitted on : Tuesday, April 1, 2008 - 3:49:34 PM
Last modification on : Thursday, December 10, 2020 - 10:52:47 AM
Long-term archiving on: : Friday, November 25, 2016 - 9:56:48 PM

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  • HAL Id : tel-00268886, version 1

Citation

Morgane Agez. ETUDE STRUCTURALE ET FONCTIONNELLE DE LA PROTEINE CHAPERON D'HISTONES ASF1. Biochimie [q-bio.BM]. Université Pierre et Marie Curie - Paris VI, 2008. Français. ⟨tel-00268886⟩

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