Abstract : Phospholipids play important roles in cell metabolism and signal transduction. In platelets, they are necessary to the development of platelet activation processes. Their hydrolysis necessitates their permanent regeneration. In the present work, we considered the possibility that phospholipids could be transferred from very-low density lipoproteins (VLDL) to platelets. Our work was organized in three stages.
Firstly, we have shown that PL transfer was stimulated by platelet activation and by the lipoprotein lipase- mediated lipolysis of VLDL.
Secondly, using a series of metabolic inhibitors we have demonstrated that the phospholipid transfer to platelets was dependent upon the activity of cytosolic phospholipase A2.
Finally, we studied the putative abnormalities of this transfer in the specific context of type 2 diabetes. We characterized several alterations that could participate to the platelet hyperactivation process observed in this pathology.