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Étude des mécanismes d'actions de la stimulation électrique haute fréquence utilisée comme traitement de la maladie de Parkinson.

Abstract : Parkinson's disease is a neurodegenerative disease of hypomotricity. Both genetic and environmental factors are involved in it development as research could state. Pharmacological or chirurgical treatments can be considered. Deep brain stimulation (DBS) developed by Professor Benabid, is a treatment that proved its efficiency. As a whole, this study intends to analyse how the DBS mechanisms are. A micro-array transcriptomic was done. It enables to highlight that electrical high frequency stimulate a deep change in the genetic expression profile. Major impacts are seen on the expression of genes responsible of protein synthesis and catabolism as well as many acting on RNA transcription and maturation. The importance of quality control is highlighted and techniques to improve reproducibility of micro-array's results are described. A second step analyse proteins' expression through incorporation of radioactive methionin and SELDI-TOF mass spectrometry. Results shown a significant decrease of proteins' expression provoked by the high frequency electrical stimulation.
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https://tel.archives-ouvertes.fr/tel-00258852
Contributor : Ludovic Alazay <>
Submitted on : Monday, February 25, 2008 - 4:32:00 PM
Last modification on : Friday, November 6, 2020 - 4:03:04 AM
Long-term archiving on: : Friday, November 25, 2016 - 8:01:54 PM

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  • HAL Id : tel-00258852, version 1

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Ludovic Alazay. Étude des mécanismes d'actions de la stimulation électrique haute fréquence utilisée comme traitement de la maladie de Parkinson.. Biologie cellulaire. Université Joseph-Fourier - Grenoble I, 2007. Français. ⟨tel-00258852⟩

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