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BOULEVERSEMENTS ARCHITECTURAUX INDUITS DANS LA MUQUEUSE COLIQUE NORMALE ET TUMORALE PAR LA TRANSFORMATION MALIGNE ET LA PROGRESSION TUMORALE :
APPROCHE MORPHOLOGIQUE

Joelle Simony 1
1 RFMQ
TIMC - Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525
Abstract : We present a morphological - based approach of architectural disorders due to malignant transformation and progression. With this aim in view, sporadic colorectal cancers were compared with non malignant (normal and dysplastic) colonic mucosa samples.First, owing to histomorphological parameters (significantly increased thickness, low density of normal crypts, an increase of abnormal crypts), normal mucosa adjacent to cancer was distinguished from distant mucosa. Yet proliferating cells marker ( Ki-67), β-catenin and Cyclin D1 immunolabelling showed no significant difference . Those data suggest a balancing mechanism to preserve normal crypt cells rather than a genuine proliferative process.Secondly, immunolabelling with Ki-67, β-catenin and Cyclin D1 antigens was done on malignant and non malignant samples, to determine cycling cells. Then, we used Voronoï's diagramm, a tool of cellular sociology, to quantify tissular architecture disorders within cycling and non cycling cells. Compared to normal mucosa cycling cells, malignant cycling cells show significant spatial rearrangement disorders, which is not the case of non cycling cells. Finally this study stresses tumor cells heterogeneity ; tumor cells heterogeneity was analyzed by " virtual spotting ", demonstrating markers-dependant labelling heterogeneity. Then, we quantified this heterogeneity, using a spatial heterogeneity index, a new mathematical tool. Dysplasic mucosa samples and well differentiated tumors demonstrate higher levels of heterogeneity than poor differentiated cancers.
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Submitted on : Tuesday, February 12, 2008 - 11:25:16 AM
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Joelle Simony. BOULEVERSEMENTS ARCHITECTURAUX INDUITS DANS LA MUQUEUSE COLIQUE NORMALE ET TUMORALE PAR LA TRANSFORMATION MALIGNE ET LA PROGRESSION TUMORALE :
APPROCHE MORPHOLOGIQUE. Biologie cellulaire. Université Joseph-Fourier - Grenoble I, 2007. Français. ⟨tel-00250911⟩

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