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Theses

Activité constitutive et adressage axonal du récepteur cannabinoïque neuronal CB1

Abstract : Constitutive activity is a pharmacological property common to numerous G Protein CoupledReceptors, but significance of this phenomenon remains unknown. This work is a study of therelationships between pharmacology and trafficking of the cannabinoid receptor CB1R, whichis expressed to large amounts in the brain and possess a reported constitutive activity. Whenexpressed in HEK-293 cells, CB1R is constitutively endocytosed and recycled, thuscontinuously cycling between plasma membrane and endosomes, due to its constitutiveactivity. At equilibrium, most CB1Rs are located in intracellular endosomes. CB1Rconstitutive endocytosis is clathrin-dependent, and intracellular transport dependent on Rab5and Rab4, but not Rab11. In cultured hippocampal neurons, this endocytosis/recycling cycleis restricted to the somatodendritic compartment, whereas axonal receptors remain stable atthe axonal plasma membrane. This cycle depends on the constitutive activity of CB1R, andconstitutive endocytosis in the somatodendritic compartment drives establishment of thepolarized surface expression of the receptor along axons.
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https://tel.archives-ouvertes.fr/tel-00250338
Contributor : Christophe Leterrier <>
Submitted on : Monday, February 11, 2008 - 4:58:10 PM
Last modification on : Monday, December 14, 2020 - 9:55:41 AM
Long-term archiving on: : Friday, September 28, 2012 - 9:30:50 AM

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  • HAL Id : tel-00250338, version 1

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Christophe Leterrier. Activité constitutive et adressage axonal du récepteur cannabinoïque neuronal CB1. Neurosciences [q-bio.NC]. Université Pierre et Marie Curie - Paris VI, 2006. Français. ⟨tel-00250338⟩

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