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Etude de l'adressage des protéines GRAs transmembranaires de Toxoplasma gondii aux granules denses et de leur insertion membranaire post-sécrétoire.

Abstract : The success of many intracellular pathogens relies on the export of both soluble and membrane-bound proteins that are destined to modify various compartments of the host cell. In Toxoplasma gondii, it is well established that the dense granules (DG) constitute the default constitutive pathway for soluble proteins. By contrast, the mechanism by which transmembrane proteins are sorted to the DG and are maintained in a soluble state while adopting a transmembrane topology after secretion is not known.
The GRA5 DG protein of T. gondii is targeted to the parasitophorous vacuole membrane (PVM) after soluble secretion. Expression of GRA5 in mammalian cells revealed that the protein is targeted to the cell surface with a type I topology, providing evidence that soluble trafficking of GRA5 within the parasite is peculiar. By using chimeric proteins containing specific domains of GRA5 and of a parasite plasma membrane (PPM) targeted transmembrane protein, we investigated which are the determinant(s) of PPM versus PVM targeting. We demonstrated that the GRA5 Nt domain is involved in soluble targeting within the DG and is essential for insertion into the PVM. These results, that were extented to another transmembrane GRA protein (GRA6), contrast with the broad acceptance that sorting signals are present within the cytoplasmic tail of membranous proteins and/or depend on the size of their transmembrane domain.
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https://tel.archives-ouvertes.fr/tel-00212284
Contributor : Claire Gendrin <>
Submitted on : Tuesday, January 22, 2008 - 4:23:42 PM
Last modification on : Friday, November 6, 2020 - 4:15:27 AM
Long-term archiving on: : Wednesday, April 14, 2010 - 10:35:48 PM

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Claire Gendrin. Etude de l'adressage des protéines GRAs transmembranaires de Toxoplasma gondii aux granules denses et de leur insertion membranaire post-sécrétoire.. Biologie cellulaire. Université Joseph-Fourier - Grenoble I, 2007. Français. ⟨tel-00212284⟩

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