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Développement de facteurs de régulation photoactivables

Abstract : Cells in a mulicellular organism constantly adjust their components concentrations in response to the interactions with their neighbors and the environment. During embryogenesis, an accurate response is of particular importance. We have developed during this thesis a technique which allows a single cell control of cellular functions in an intact organism. The combined used of caged molecules and two-photon excitation allowed us to fulfill this goal.
We first discuss on the requirements and the protecting groups properties needed to use such a tool in a biological context. Then, we relate on the characterization of the two-photon absorption properties of the protecting groups which have been used throughout this work. Finally, we apply this technique to retinoic acid signaling in zebrafish. We show that we can deliver any well defined concentration of molecules in a single cell with second temporal resolution in an intact embryo. Using that, we can study the dynamics of this signaling pathway and evidence a cell-autonomous fast negative control of it and we identify MAP kinase p38 as being necessary to this phenomenon.
We also show in the appendices the proof of principle of caged compounds use to trigger genomic recombination in zebrafish and to inhibit enzymatic function (topoisomerase activity in this case).
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Contributor : Pierre Neveu <>
Submitted on : Thursday, January 10, 2008 - 7:00:41 PM
Last modification on : Thursday, December 10, 2020 - 12:38:38 PM
Long-term archiving on: : Tuesday, April 13, 2010 - 5:00:31 PM


  • HAL Id : tel-00203671, version 1


Pierre Neveu. Développement de facteurs de régulation photoactivables. Biophysique []. Université Paris Sud - Paris XI, 2007. Français. ⟨tel-00203671⟩



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