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Sondes fluorescentes pour l'ADN : marquages covalent et non-covalent.

Abstract : Fluorescent labelling of biomolecules has become increasingly important for biological studies. In this context, we designed new probes for covalent and non-covalent labelling of DNA.
The non covalent approach was based on structural recognition principles and made use of G4-DNA as target. The mixed properties of conjugates comprised of a G4-binder motif and of a fluorescent label prompted us to use Thiazole Orange (TO). TO binds G4-DNA with a high affinity and exhibits a strong fluorescence exaltation. Thus, affinities of G4-binders can be evaluated by competitive displacement of this probe. It was also shown that G4-DNA represents a scaffold for energy transfer (FRET) between a G4-binder and TO.
The covalent approach was based on the design of small-sized derivatizable fluorescent probes with two photon absorption properties. Some of these derivatives show a strong fluorescent exaltation in presence of DNA and two photon absorption properties stronger than usual biological dyes.
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Submitted on : Sunday, January 6, 2008 - 2:24:20 PM
Last modification on : Friday, March 5, 2021 - 3:11:27 PM
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  • HAL Id : tel-00202422, version 1

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Clémence Allain. Sondes fluorescentes pour l'ADN : marquages covalent et non-covalent.. Autre. Université Pierre et Marie Curie - Paris VI, 2006. Français. ⟨tel-00202422⟩

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