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Impression de biomolécules par lithographie douce, applications pour les biopuces, de l'échelle micrométrique

Abstract : The main purpose of this research work is the demonstration that soft-lithography, very often called Micro-Contact Printing (µCP) is an efficient patterning technique for arranging biomolecules on a surface in the perspective of biochip applications. For DNA Micro-arrays applications, we demonstrate that µCP is a competitive method compared to the conventional spotting technology, commonly used today. The cost of the technology is much lower, the surface density of the chip is drastically increased and the quality and definition of the biopatterns are greatly improved. A systematic study of the inking mechanisms of the elastomeric stamps is provided together with the study and comprehension of transfer mechanisms of molecules from the surface of the stamp to the substrate. The crucial role played by the free fragments of polymers not cross-linked during the polymerisation of the stamp is highlighted. In a second section we investigate the possibility of using µCP for generating single biomolecule biochips. We show how this printing technique can be optimized for reaching sub-micrometric scale down to 50 nanometers features. Two technological processes involving soft-lithography are proposed: the first one for combing long DNA molecules on spatially organized and registered positions for genetic applications, the second one for assembling individual DNA molecules in ordered arrays compatible with the dynamic tracking of individual DNA molecules over large populations.
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Submitted on : Thursday, December 20, 2007 - 11:09:51 AM
Last modification on : Thursday, June 10, 2021 - 3:03:55 AM
Long-term archiving on: : Friday, November 25, 2016 - 8:54:11 PM

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  • HAL Id : tel-00200042, version 1

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Christophe Thibault. Impression de biomolécules par lithographie douce, applications pour les biopuces, de l'échelle micrométrique. Micro et nanotechnologies/Microélectronique. INSA de Toulouse, 2007. Français. ⟨tel-00200042⟩

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