Skip to Main content Skip to Navigation
Theses

Le vaccin antivariolique historique Lister : séquence génomique, diversité phénotypique et neuropathogénicité : perspectives vaccinales

Abstract : The smallpox constituted a real scourge for humanity until 1980, the year of the declaration of its eradication due to vaccination. The risk of variola virus reemerging as a biological weapon, considering manufacturing conditions, the low population immunity and historical vaccine post-vaccinal complications, make new smallpox vaccines development a necessity.
In this paper, the historical Lister strain vaccine study is described as part of the elaboration strategy of a new vaccine coming from this strain.
The genomic sequence of this vaccine is determined and described. Its comparison with other vaccinia virus strains underlines the Lister strain singularities.
The viral population diversity study as well as the phenotypic and genetic characterization should highlight strategy choice for the development of a new second generation vaccine coming from the Lister strain : a clonal vaccine or a polyclonal one.
Finally, the study of the vaccinia virus interaction with the blood-brain barrier demonstrates an increase in permeability due to vaccinia virus endothelial cells infection. Moreover, in vivo studies exhibit the viral entry and replication in the brain, sometimes responsible for critical cerebral reaches. These results suggest the first generation smallpox vaccine neuropathogenesis could have caused deadly post-vaccinal encephalitises, smallpox vaccination complications.
Document type :
Theses
Complete list of metadata

https://tel.archives-ouvertes.fr/tel-00198642
Contributor : Aude Garcel <>
Submitted on : Wednesday, December 19, 2007 - 10:21:25 AM
Last modification on : Wednesday, September 27, 2017 - 1:07:00 AM
Long-term archiving on: : Monday, April 12, 2010 - 8:12:25 AM

Identifiers

  • HAL Id : tel-00198642, version 1

Collections

UJF | SSA

Citation

Aude Garcel. Le vaccin antivariolique historique Lister : séquence génomique, diversité phénotypique et neuropathogénicité : perspectives vaccinales. Autre [q-bio.OT]. Université Joseph-Fourier - Grenoble I, 2007. Français. ⟨tel-00198642⟩

Share

Metrics

Record views

485

Files downloads

4907