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Les interactions multivalentes : leurs rôles dans les processus de reconnaissance biomoléculaire et leur application dans la construction d'assemblage supramoléculaire

Abstract : This work deals with a quantitative study of several biomolecular recognition systems involving multivalent interactions.
Two chapters focuse on the use of supramolecular cyclodecapeptidic platform called RAFT (Regioselectively Addressable Functionnalized Template), which allows the presentation of multiple carbohydrate or cyclopeptidic ligands. Kinetic and thermodynamic studies of the interaction between the ligands RAFT-carbohydrate and a model lectin, concanavalin A, have demonstrated that two molecular mechanisms are responsible for the better affinity of multivalent RAFT molecule compared to their monovalent counterparts: on one hand a "proximity-statistical" effect due to the high local concentration of sugar entities and on the other hand thanks to a "cluster effect" which confers the ability of multivalent RAFT to bind several lectins. Preliminary studies have also involved the analysis of the interaction between RAFT-RGD and integrin cell receptors.
In a last chapter, we have demonstrated, for the first time, polymer multilayer formation based on host-guest interaction between two derivatized chitosans biopolymers, one, with Β-cyclodextrin cavities and the other with adamantyl moieties. While stability of the self-assembly is conferred by multivalent complexation occuring at each step of the construction, the assembly growth is mainly governed by the availability of the complexation sites offered by each layer. Moreover, the two positively charged polymers confer to the assembly swelling/deswelling properties in response to changes in ionic strength and pH.
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https://tel.archives-ouvertes.fr/tel-00196867
Contributor : Marie Wilczewski <>
Submitted on : Thursday, December 13, 2007 - 4:43:51 PM
Last modification on : Friday, November 6, 2020 - 3:54:09 AM
Long-term archiving on: : Monday, April 12, 2010 - 7:25:54 AM

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Marie Wilczewski. Les interactions multivalentes : leurs rôles dans les processus de reconnaissance biomoléculaire et leur application dans la construction d'assemblage supramoléculaire. Autre. Université Joseph-Fourier - Grenoble I, 2007. Français. ⟨tel-00196867⟩

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