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Theses

Développement de supports polythiophène conducteurs pour l'immobilisation d'ADN, la détection électronique de l'hybridation et la libération locale de gènes

Abstract : To diagnose genetic alteration and to redress defective genes responsible for proteins deficiencies, simple sequence-specific tools are needed for immobilization of oligonucleotides, to detect genetic disorder and to release a normal gene at a specific location.
Herein, we report on an unprecedented case of a functionalized polythiophene matrix modified with an aryl sulfonyl chloride linker group suitable for the covalent immobilization of 5'-amino-terminated oligonucleotides. The use of a conducting polymer as support allows the label-free detection of the hybridization only from the changes of conductivity under the recognition process. Furthermore, the use of an electrosensitive linker group permits the subsequent release of gene upon an electric pulse.
In practice, we have immobilized a single strand 42-mer as DNA probe sequence. Next, a single strand 675-mer was used as DNA target, in which the 5' extremity is complementary to the DNA probe. The direct label-free detection of the hybridization is investigated by electrochemical impedance spectroscopy and by quartz crystal microbalance. Finally, an electrochemical stimulus leads to the release on demand of the DNA previously immobilized. The amount delivery permits to do a PCR amplification.
In recent years, there is a great interest in development of controlled drug delivery devices for release biological vectors at a specific location. Numerous strategies have been examined based on progress in material chemistry, but electrochemical probe as controlled delivery systems appears to be a promising approach that mimics better the way in which some potent drugs are release in human body.
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https://tel.archives-ouvertes.fr/tel-00192830
Contributor : Christelle Gautier <>
Submitted on : Thursday, November 29, 2007 - 3:00:04 PM
Last modification on : Friday, July 3, 2020 - 4:46:27 PM
Long-term archiving on: : Monday, April 12, 2010 - 5:31:53 AM

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  • HAL Id : tel-00192830, version 1

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Christelle Gautier. Développement de supports polythiophène conducteurs pour l'immobilisation d'ADN, la détection électronique de l'hybridation et la libération locale de gènes. Matériaux. Université du Maine, 2006. Français. ⟨tel-00192830⟩

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