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STRATEGIES D'OMOGENEISATION DES POPULATIONS DE PROGENITEURS NERVEUX FOETAUX HUMAINS DANS UNE PERSPECTIVE DE THERAPIE CELLULAIRE DU SYSTEME NERVEUX CENTRAL

Abstract : In a therapeutic purpose, human fetal neural stem cells may be the most suitable tool for the treatment of central nervous system injuries or neurodegenerative disorders. Until now, their use in cell-based therapy has appealed to heterogeneous populations consisting of immature cells, committed progenitors and fully mature cells. However, recent studies have revealed the interest of enriching the populations in a specific cellular type in order to improve graft efficiency. In our study, two different strategies were developed to homogenize human fetal progenitor populations. In the first one, neuronal differentiation was achieved by lentiviral vector-mediated overexpression of 4 proneural genes: Neurogenine 1, 2, 3 and Mash1. In the second one, lentiviral vectors were used to promote reporter protein expression under the control of lineage-specific promoters: the Nestine promoter for immature cells; the human Synapsine promoter for neurons and the GFAP promoter for astrocytes. Although hSYN and GFAP promoters were shown to be non-specific, the Nestine promoter drove GFP-expression in a majority of nestine-positive cells. These two methods should allow a selection of the desired populations. A larger-scale project should now evaluate the advantage of grafting homogeneous populations.
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https://tel.archives-ouvertes.fr/tel-00184239
Contributor : Jacques Mallet <>
Submitted on : Tuesday, October 30, 2007 - 5:22:11 PM
Last modification on : Wednesday, December 9, 2020 - 3:08:14 PM
Long-term archiving on: : Monday, April 12, 2010 - 1:02:19 AM

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  • HAL Id : tel-00184239, version 1

Citation

Angéline Serre. STRATEGIES D'OMOGENEISATION DES POPULATIONS DE PROGENITEURS NERVEUX FOETAUX HUMAINS DANS UNE PERSPECTIVE DE THERAPIE CELLULAIRE DU SYSTEME NERVEUX CENTRAL. Neurosciences [q-bio.NC]. Université Pierre et Marie Curie - Paris VI, 2007. Français. ⟨tel-00184239⟩

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