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CARACTERISATION DES PROTEINES DE GRANULES DENSES DE TOXOPLASMA GONDII :Etude des interactions protéiques et lipidiques et du rôle des hélices alpha-amphipatiques de GRA2

Abstract : At the interface with the host cell compartment, the parasitophorous vacuole of the intracellular parasite Toxoplasma gondii comprises several membranous systems, including the membranous nanotubular network formed upon secretion of the dense granule protein GRA2. Using biochemical approaches, I have shown that the GRA proteins form oligomeric complexes, which could explain the solubility of these membrane proteins within the dense granules and after secretion into the vacuolar soluble fraction. Using molecular and cellular approaches, I have investigated the involvement of each of the three GRA2 amphipathic alpha-helices in post-secretory membrane association of the protein, likely via association to phosphatidylinositol phosphates, and in the GRA2 induced-formation of the membranous nanotubular network. These studies led us to propose models for the interaction of GRA2 with membranes and for the tubulation of the membranous nanotubular network.
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https://tel.archives-ouvertes.fr/tel-00182730
Contributor : Laetitia Travier <>
Submitted on : Friday, October 26, 2007 - 8:51:40 PM
Last modification on : Friday, November 6, 2020 - 4:07:14 AM
Long-term archiving on: : Monday, April 12, 2010 - 12:51:04 AM

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  • HAL Id : tel-00182730, version 1

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Laetitia Travier. CARACTERISATION DES PROTEINES DE GRANULES DENSES DE TOXOPLASMA GONDII :Etude des interactions protéiques et lipidiques et du rôle des hélices alpha-amphipatiques de GRA2. Biologie cellulaire. Université Joseph-Fourier - Grenoble I, 2007. Français. ⟨tel-00182730⟩

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