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Contrôle de la vitesse de germination chez le maïs (Zea mays) : étude de la voie de biosynthèse des acides aminés issus de l'aspartate et recherche de QTLs

Abstract : The aspartate pathway was studied through the expression of the genes ask1, ask2 akh1 and akh2 coding for aspartate kinase by qt-RT-PCR, the amino acids content by HPLC and the degradation of asp-15N in the derived amino acids followed by labelling of 15N. It appears that the aspartate pathway differs between two (slow and fast) genotypes of maize: slow germination would be associated with an inhibiting effect of the accumulation of lysine or to limited contents of threonine and methionine. The study of the inhibitory effect of lysine by QTLs (T50) in the presence of amino acids highlights a QTL specific to germination in the presence of lysine, which coincides with a QTL specific to germination in condition of "cold" stress. The results indicate that lysine content would be partly responsible for the speed of germination.
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https://tel.archives-ouvertes.fr/tel-00181021
Contributor : Fabiola Anzala <>
Submitted on : Monday, October 22, 2007 - 6:45:50 PM
Last modification on : Saturday, June 6, 2020 - 9:55:34 PM
Long-term archiving on: : Tuesday, September 18, 2012 - 11:56:19 AM

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  • HAL Id : tel-00181021, version 1
  • PRODINRA : 251171

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Fabiola Anzala. Contrôle de la vitesse de germination chez le maïs (Zea mays) : étude de la voie de biosynthèse des acides aminés issus de l'aspartate et recherche de QTLs. Biochimie [q-bio.BM]. Université d'Angers, 2006. Français. ⟨tel-00181021⟩

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