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Homéostasie et résistance au cuivre chez Cupriavidus metallidurans CH34 : la protéine CopH et les transporteurs membranaires CusA et CzcA

Véronique Sendra 1
1 LPM - Laboratoire des Protéines Membranaires
IBS - UMR 5075 - Institut de biologie structurale
Abstract : The copH gene is one of the 19 genes found in the cop cluster, involved in detoxification of copper from the cytoplasme as well as from the periplasm, in Cupriavidus metallidurans CH34, used as a model to study heavy metals resistance in bacteria. The function of CopH protein, located in the periplasm, is not clear yet, but its expression is induced by copper. We analysed the copper binding properties of CopH. The features are consistent with the presence of Cu(II) type 2 centers in a nitrogen ligand field. The only two histidine residus, ligands of copper as we thought, would not interact directly avec Cu(II) ions. The copper site would consist with 45 nitrogen atoms from the backbone of CopH.
The study of membrane transporters CusA and CzcA has needed the optimised purification in detergents and the analysis of their behaviour in solution.
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Submitted on : Friday, October 19, 2007 - 2:54:28 PM
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Véronique Sendra. Homéostasie et résistance au cuivre chez Cupriavidus metallidurans CH34 : la protéine CopH et les transporteurs membranaires CusA et CzcA. Biochimie [q-bio.BM]. Université Joseph-Fourier - Grenoble I, 2007. Français. ⟨tel-00180556⟩

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