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Theses

Contrôle et analyse électrochimique de la réactivité biologique à l'échelle de la cellule unique dans un dispositif microfluidique

Abstract : For the analytical study of single cells or small groups of cells, it is of the utmost importance to reduce the sample volume and therefore to increase both the local concentration of the analytes and the sensitivity of the measurements. Micro-analytical systems are particularly relevant for such purposes. This thesis presents a novel analytical tool, coupling the dynamic control of micro-environments with microfluidics and the instant and local analysis of electroactive molecules with
electrochemistry.
With the development of convenient microfluidic cell culture tools and cell manipulation schemes, we have set up a stable and long-term dynamic control of chemical stimulations on a population of Mesenchymal Stem Cells (MSC). We are striving towards the induction of MSC's biological reactivity into the neuronal differentiation pathway.
To demonstrate an on-chip electrochemical detection of oxydative stress on single cells, we have fabricated a sophisticated microsystem with an array of detection chambers, each addressed by a microelectrode, and in which macrophage cells can be trapped and maintained alive throughout the detection time. By varying the detection conditions, the number of cells inves-
tigated in the same micro-environment, the type of stimulations or the degree of intercellular communications between the objects investigated, we are establishing the building blocks for an unparalleled analytical study that has yet to be realized.
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https://tel.archives-ouvertes.fr/tel-00178656
Contributor : Cécile Crozatier <>
Submitted on : Thursday, October 11, 2007 - 6:44:20 PM
Last modification on : Monday, December 14, 2020 - 9:50:24 AM
Long-term archiving on: : Monday, September 24, 2012 - 1:20:57 PM

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  • HAL Id : tel-00178656, version 1

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Cécile Crozatier. Contrôle et analyse électrochimique de la réactivité biologique à l'échelle de la cellule unique dans un dispositif microfluidique. Autre. Université Paris-Diderot - Paris VII, 2007. Français. ⟨tel-00178656⟩

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