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Développement de systèmes membranaires modèles pour la vacuole parasitophore de Toxoplasma gondii :
Interactions des protéines de granules denses (protéines GRA) avec des vésicules unilamellaires

Abstract : GRA proteins are secreted by the intracellular parasite Toxoplasma gondii into the parasitophorous vacuole, where most of them interact with two systems of tubular membranes : the Host Organelle Sequestering Tubules (HOSTs) and the Membrane Nanotubules Network (RNM). Although most of the GRA proteins contain potential transmembrane domains, they are secreted as soluble forms and become membrane-associated only when they reach their target membranes. This unusual property led to consider them as attractive models of protein-membrane interactions. I developed two experimental approaches to study the interactions of GRA proteins, extracted from the parasite or from the vacuole, with model membranes. Firstly, biochemical approaches using Small Unilamellar Vesicles (SUVs) led to characterize the solubilisation forms of GRA proteins and their association with SUVs membranes. Secondly, I developed a Giant Unilamellar Vesicles (GUVs) model to study the interactions of GRA proteins with membranes by fluorescence spectroscopy methods. The results provide elements 1) which help to decipher the traffic of GRA proteins within the parasite and the PV, and 2) which open the way to set up an in vitro minimal system to study the building up of the parasitophorous vacuole and of its associated tubular membranes.
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https://tel.archives-ouvertes.fr/tel-00177965
Contributor : Pauline Ruffiot <>
Submitted on : Tuesday, October 9, 2007 - 6:24:26 PM
Last modification on : Tuesday, September 11, 2018 - 11:00:02 AM
Long-term archiving on: : Sunday, April 11, 2010 - 10:38:02 PM

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  • HAL Id : tel-00177965, version 1

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Pauline Ruffiot. Développement de systèmes membranaires modèles pour la vacuole parasitophore de Toxoplasma gondii :
Interactions des protéines de granules denses (protéines GRA) avec des vésicules unilamellaires. Biophysique [physics.bio-ph]. Université Joseph-Fourier - Grenoble I, 2007. Français. ⟨tel-00177965⟩

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